碱蓬水溶性多糖SPB的分离纯化以及活性的初步研究  

Studies on separation,purification,and activities of a water-soluble polysaccharide SPB from Suaeda Spp

作　　者：王昭晶[1] 

机构地区：[1]华侨大学生物工程与技术系,厦门361021

出　　处：《食品科技》2010年第9期236-238,共3页Food Science and Technology

基　　金：华侨大学科研基金资助项目(08X0212);国务院侨办基金资助项目(07QZR07)

摘　　要：目的:从碱蓬中提取出水溶性粗多糖SP,经分离纯化得到SPB,研究SPB的纯度,性质和体外抗氧化能力。方法:粗多糖经DEAE Sepharose CL-6B柱层析分离纯化得到SPB,经HPLC法检验纯度,超氧阴离子清除实验和羟自由基清除实验等鉴定其抗氧化能力。结果:SPB是均一组分多糖,分子量在40万。结论:SPB有高于Vc的超氧阴离子清除作用,以及相当于Vc的羟自由基清除作用。A water-soluble crude polysaccharide SP was obtained from Suaede Spp.After crude polysaccharide was fractionated and purified by DEAE Sepharose CL-6B column chromatography,SPB was obtained.The homogeneity was proved by HPLC method.SPB were homogeneous and.On the basis of antioxidant activities assay,SPB has stronger inhibitory activities for superoxide radical than Vc.

关 键 词：碱蓬 纯化 抗氧化活性 

分 类 号：Q539[生物学—生物化学]