脂肪酶合成聚乙二醇_(400)油酸单酯和双酯的影响因素  

Factors Influencing the Synthesis of Polyethelene Glycol 400 Oleate Mono and Diester by Lipase

作　　者：寇秀芬[1] 徐家立[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》1999年第3期301-303,共3页Chinese Journal of Biotechnology

摘　　要：研究了固定化假丝酵母（Ｃａｎｄｉｄａｓｐ．）１６１９脂肪酶合成聚乙二醇４００油酸单酯与双酯的影响因素。不同摩尔比的底物反应６ｈ都有单酯和双酯生成。酸与醇的摩尔比为０２５∶１到２∶１时，生成单酯与双酯的比例在３５∶１到４∶１的范围内；当酸与醇的摩尔比达到３∶１至８∶１时，单、双酯的生成量相仿。反应达平衡时（２４ｈ），不同摩尔比底物的反应产物都是双酯。在含己烷的反应体系中，反应平衡时有单酯存在，摩尔比为２∶１时，反应物中单、双酯比达到１∶３２。The factors influencing the synthesis of polyethelene glycol 400 oleate mono and diesters by immobilized lipase from Candida sp. 1619 were investigated.Mono and diesters were formed after 6h reaction with different molar ratio of the substrates.The ratio of monoester to diester formed was in the ranging of 3.5∶1 to 4∶1,when the molar ratio of acid to PEG 400 was from 0.25∶1 to 2∶1.Almost equal amounts of mono and diesters were produced when the molar ratio of acid to PEG 400 was 3∶1 to 8∶1.Only diester was found in reaction mixture with different molar ratio of the substrates when the equilibrium of reaction have been reached (22h).The ratio of mono to diester was 1∶3.2 in the reaction system containing hexane even the molar ratio of substrates was 2∶1.

关 键 词：固定化 脂肪酶 聚乙二醇400 油酸单酯 双酯 合成 

分 类 号：TQ423.21[化学工程]