应用雾化营养液生物反应器繁殖脱毒马铃薯的研究  

Propagation of Virus-free Potato Using a Nutrient Mist Bioreactor

作　　者：郝震龙[1] 欧阳藩[1] 耿玉轩[2] 邓向东[2] 胡赞民[2] 陈正华[2] 

机构地区：[1]中国科学院化工冶金研究所生化工程重点实验室 [2]中国科学院遗传研究所305课题组

出　　处：《生物工程学报》1999年第3期355-361,共7页Chinese Journal of Biotechnology

摘　　要：利用雾化营养液生物反应器（简称“雾化反应器”）对马铃薯（Ｓｏｌａｎｕｍｔｕｂｅｒｏｓｕｍ）苗的培养和微型薯的诱导进行研究。分析了苗生长过程中生物量、营养雾传递和糖消耗的变化以及微型薯诱导的时间进程。培养结果表明：与液体或固体培养基相比，雾化反应器培养能提高苗的繁殖效率和促进微型薯的诱导。采用两步法提高了雾化反应器诱导微型薯的效果。Shoot culture and microtuber induction of potato( Solanum tuberosum )in a nutrient mist bioreactor(NMB) were studied.Changes of biomass,nutrient mist transfer and sugar consumption during the shoot culture and time course of tuber induction were investigated.Results showed that,compared with liquid or solid medium,the nutrient mist bioreactor improved the efficiency of shoot propagation and promoted microtuber induction.Using two-step method improved the effects of NMB on microtuber induction.

关 键 词：生物反应器 营养雾培养 马铃薯 苗培养 

分 类 号：S532.035.3[农业科学—作物学]