重组人肿瘤坏死因子α(hTNFα)衍生物发酵条件的研究  

Fermentation process of recombinant human tumor necrosis factor(hTNFα)derivative

作　　者：徐春晓[1] 倪道明[1] 张振龙[1] 王春荣[1] 樊晓翔[1] 邓婉明 

机构地区：[1]卫生部北京生物制品研究所

出　　处：《微生物学免疫学进展》1999年第3期36-40,共5页Progress In Microbiology and Immunology

摘　　要：本文对一种新型重组人肿瘤坏死因子衍生物（命名为ｈＴＮＦＤ）的发酵条件进行了初步研究。通过实验优化选择了适宜该衍生物表达的培养基、ＩＰＴＧ使用浓度、诱导时期以及诱导时间的长短等，并在５０Ｌ发酵罐进行了中试规模的放大培养，菌体的收获量湿重可达１６４３ｇ／Ｌ，ｈＴＮＦＤ表达量占总蛋白的６０％，纯化的衍生物比活为１０１×１０１０Ｕ／ｍｇ，比原型ｈＴＮＦα提高了４６５倍，具有潜在的临床应用价值。Fermentation process of a novel recombinant human tumor necrosis factor α(hTNFα)derivative(hTNFD) was studied Expression medium,IPTG concentration,induction time and induction period were optimized.Fermentation process was scaled up to pilot scale on 50 L fermenter.The yield of hTNFD could reach 16.43 g/L and expression level of hTNFD was 60%.Antitumor activity of purified hTNFD was 1.01×10 10 U/mg and 465 times higher than that of natural type hTNFα,which displayed a potential therapeutic application.

关 键 词：肿瘤坏死因子 衍生物 发酵 重组 

分 类 号：Q784[生物学—分子生物学] TQ464.7[化学工程—制药化工]