黄曲霉毒素直接竞争法ELISA试剂盒的研制及其在花生和花生制品中的应用  

Application of the directness competition ELISA kit for determination aflatoxin content in peanut and peanut products

作　　者：王雄 王艳斐 果旗 荣钊 陈冰君 王丽[2] 吴继宗[3] 

机构地区：[1]北京中检维康技术有限公司 [2]长江大学生命科学学院 [3]中国原子能科学研究院

出　　处：《中国饲料》2010年第16期37-39,共3页China Feed

摘　　要：本实验基于在黄曲霉毒素单克隆抗体基础上,建立酶联免疫(ELISA)直接法检测花生及其制品中黄曲霉毒素(B1、B2、G1、G2)的含量。应用黄曲霉毒素抗体和酶标记的黄曲霉毒素建立酶联免疫直接竞争法。试剂盒最低检出浓度为0.5ng/mL,标准曲线的线性范围为0.5～10.0ng/mL;对花生和花生粕的添加回收率为70.0%～110.0%,变异系数小于20%。本实验制备的黄曲霉毒素试剂盒能够应用于花生及其制品的检测,酶联免疫直接法是一种简单、快速、灵敏、准确的检测方法。Based on anti-aflatoxins monoclonal antibody,the directness competition ELISA method was developed, which was used to detect the level of contamination of aflatoxins （B1,B2,G1,G2）in peanut and peanut products.Application of anti-aflatoxins monoclonal antibody and HRP markers of aflatoxins,the directness competition ELISA method was developed.For the standard aflatoxins,the limit of detection was 0.5 ng/mL.The calibration curve of coating second antibody with standard aflatoxins,the linear range was 0.5～10.0 ng/mL.The recovery rates of spiked peanut and peanut cake were between 70.0 % and 110.0 %,the coefficient of variation （CV） was below 20 %.The directness competition ELISA for determination aflatoxins content in peanut and peanut products was a simple,rapid,sensitive and accurate method.

关 键 词：酶联免疫吸附试验 黄曲霉毒素 单克隆抗体 花生 

分 类 号：S816.17[农业科学—饲料科学]