枣缩果病果实内微生物种群多样性的PCR-DGGE分析  

Diversity Analysis of Microbial Community from the Fruit of Jujube Fruit Shrink Disease by PCR-DGGE

作　　者：侯晓杰[1] 崔建州[1] 李正楠[2] 于占晶[1] 牛晓科[1] 冉隆贤[1] 

机构地区：[1]河北农业大学林学院,河北保定071001 [2]河北农业大学山区研究所,河北保定071000

出　　处：《中国食品学报》2010年第4期260-266,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：教育部科学技术研究重点项目(No.208017);河北省自然基金项目(No.C2010000675)

摘　　要：采用聚合酶链式反应(PCR)技术,以阜平大枣枣缩果病果实病部、阜平大枣健康果实抽提到的组织总DNA为模板,对其细菌16S rDNA的V3可变区及真菌18S rDNA进行扩增,并对扩增产物进行DGGE指纹图谱分析,研究阜平大枣缩果病果实与健康果实内的微生物种群变化。试验结果表明,PCR-DGGE法是研究枣果内微生物组成的可行方法;阜平大枣缩果病果实内细菌及真菌菌群种类较健康果实减少,有些细菌及真菌的优势菌在病果样品内不存在,健康样品内的某些优势菌在病部为非优势菌。The V3 variable fragment for 16S rDNA of bacteria and the variable fragment for 18S rDNA of fungi were amplified from the genome DNA by polymerase chain reaction（PCR）.The DNA extracted from the healthy fruit of Zizyphus jujuba var.Fupingdazao and the fruit of jujube fruit shrink disease.The diversity of microbial floras of Zizyphus jujuba var.Fupingdazao was studied by PCR-DGGE.The results showed that the PCR-DGGE technology was a feasible method for analysis of microbial composition in Zizyphus jujuba var.Fupingdazao.The population of endophytic bacteria and endophytic fungi from the diseased friut were fewer than the ones from the healthy friut,and some of predominant bacterial floras in healthy friut didn＇t exesit in the diseased friut,and some of them became non-dominant floras.

关 键 词：阜平大枣 内生细菌 内生真菌 变性梯度凝胶电泳 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]