毫米波辐射对兔眼房水一氧化氮及某些细胞因子的影响  

Influence of nitric oxide and cytokine in rabbit aqueous humor by millimeter wave radiation

作　　者：王小娟[1,2,3] 王多宁[1,2,3] 王晓莉[1,2,3] 李缉熙 李琦[1,2,3] 马吉献 

机构地区：[1]第四军医大学西京医院 [2]第四军医大学 [3]西安电子科技大学电磁工程系

出　　处：《中华物理医学与康复杂志》1999年第1期6-8,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金

摘　　要：目的观察毫米波辐射后兔眼房水一氧化氮（ＮＯ）及白细胞介素６（ＩＬ６）、白细胞介素８（ＩＬ８）、肿瘤坏死因子（ＴＮＦα）含量的变化，探讨毫米波对眼的损伤机制，为制定毫米波辐射的卫生标准提供实验依据。方法新西兰兔按受辐照后不同取材时间及辐射剂量分组，采用３５ＧＨｚ等幅连续波，辐射兔眼１ｈ，用硝酸酶还原法测定房水ＮＯ，用ＥＬＩＳＡ法测定ＩＬ６、ＩＬ８、ＴＮＦα。结果１０ｍＷ／ｃｍ２毫米波辐射结束后６ｈ，房水ＮＯ含量升高（Ｐ＜０．０１），此效应可持续２４ｈ以上；３５ｍＷ／ｃｍ２及１０ｍＷ／ｃｍ２毫米波辐射结束后２４ｈ内，未发现房水ＩＬ６、ＩＬ８、ＴＮＦα含量的升高。结论低功率３５ＧＨｚ毫米波辐射可引起房水中ＮＯ含量的增高。Objective To observe the changes of NO and cytokine content in rabbit aqueous humor followed millimeter wave radiation and to offer the experimental basis for determination of damaging threshold of millimeter wave radiation.Methods New Zealand rabbits were divided into groups according to different sampling time and radiated dosage.The eyes of the rabbits were radiated with 35GHz millimeter wave for one hour.Nitrate determination in aqueous humor was conducted by enzymatic assay with nitrate reductase.IL 6,IL 8 and TNF α in aqueous humor were quantitatively measured by ELISA.Results Early in 6 hours following exposure with the power density of 10 mW/cm 2,NO content was increased and later persisted for over 24 hours ( P < 0.01 ).IL 6,IL 8 and TNF α were not changed in 24 hours following radiation with 35 mW/cm 2 and 10 mW/cm 2.Conclusion NO content in aqueous humor is increased by low power millimeter wave radiation,indicating NO is one of the important factors in the cornea injury by millimeter wave.

关 键 词：辐射损伤 毫米波 眼损伤 一氧化氮 眼房水 

分 类 号：R779.12[医药卫生—眼科] R312[医药卫生—临床医学]