武汉地区十二指肠溃疡患者和HLA-DQA1基因关联的初步研究  

HLA DQA1 genes involved in the genetic susceptibility to duodenal ulcer in Wuhan Hans

作　　者：杜意平[1,2] 邓长生[1,2] 鲁德银[1,2] 黄梅芳 郭淑芳[1,2] 侯伟 

机构地区：[1]湖北医科大学第二医院消化内科 [2]深圳市中心医院消化内科

出　　处：《中华医学遗传学杂志》1999年第2期85-87,共3页Chinese Journal of Medical Genetics

摘　　要：目的探讨武汉地区汉族人ＨＬＡ－ＤＱＡ１基因与十二指肠溃疡的遗传关联。方法应用ＨＬＡ基因的聚合酶链反应－限制性片段长度多态性核苷酸分型技术，以等位基因特异性的限制性内切酶（ＡｐａｌⅠ、ＢｓａｊⅠ、ＨｐｈⅠ、ＦｏｋⅠ、ＭｂｏⅡ、ＭｎｌⅠ）消化ＤＱＡ１座位特异的ＰＣＲ扩增产物，对７０例十二指肠溃疡患者、５０例正常人的ＨＬＡ－ＤＱＡ１等位基因进行分析。结果十二指肠溃疡患者ＤＱＡ１＊０３０１等位基因频率与正常对照组明显增高，差异有显著性（Ｐ＝０．００３，ＲＲ＝３．２０，Ｐｃ＝０．０２４）。结论ＤＱＡ１＊０３０１与十二指肠溃疡有一定关联，十二指肠溃疡患者与正常人之间存在着免疫遗传异质性的差异。Objective To study the genetic susceptibility of HLA DQA1 alleles to duodenal ulcer in Chinese Hans from Wuhan and its nearby regions.Methods Seventy patients with duodenal ulcer and fifty healthy controls were examined for HLA DQA1 genotypes. HLA DQA1 typing was carried out by digesting the locus specific polymerase chain reaction amplified products with alleles specific restriction enzymes (PCR RFLP),Apal Ⅰ,Basj Ⅰ,Hph Ⅰ,Fok Ⅰ,Mbo Ⅱ and Mnl Ⅰ.Results The allele frequency of DQA1 0301 in patients with duodenal ulcer(64.3%) was significantly higher than that in healthy controls (36%). In contrast, the allele frequency of DQA1 0102 in patients with duodenal ulcer (8.6%) was significantly lower than that in healthy controls(26%).Conclusion These findings suggest that DQA1 0301 is a susceptibic gene for duodenal ulcer in Wuhan Hans while DQA1 0102 is its resistant gene, and there are immunogenetic differences in HLA DQA1 locus between duodenal ulcer patients and healthy controls.

关 键 词：十二指肠溃疡 HAL-DQA1基因 PCR-RFLP 

分 类 号：R573.102[医药卫生—消化系统]