氨基酸含量与寒热药性相关性的研究与统计分析  

Correlations Between Amino Acid Contents and Cold or Hot Nature of Traditional Chinese Material Medica

作　　者：冯帅[1] 李峰[1] 周正礼[1] 李健[1] 

机构地区：[1]山东中医药大学药学院,济南250355

出　　处：《中国实验方剂学杂志》2010年第11期91-95,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划("973"计划)项目(2007CB51601)

摘　　要：目的:初步探讨中药寒热药性与18种氨基酸含量的相关性,建立寒热药性的Fisher判别函数。方法:采用反相高效液相色谱法、紫外分光光度法测定了30味中药中的18种氨基酸含量。以18种氨基酸含量为指标,利用Fisher方法建立判别函数,研究中药药性与氨基酸含量的相关性。结果:热性中药的18种氨基酸平均含量是寒性中药的1.32倍,建立寒热药性的Fisher判别函数,组内回代一致率为90%。结论:Fisher判别函数对30种中药的寒热药性实现了较为准确的判别。18种氨基酸含量与中药寒热药性具有相关性。Objective：The aim of the paper was to study the relationship between 18 kinds of amino acid contents and cold or hot nature of traditional Chinese material medica and establish discriminant function.Method：Extracting eighteen kinds of amino acid from thirty kinds of TCM.Seventeen kinds of amino acid contents were determined by high performance liquid chromatography（HPLC） with precolumn phenylisothiocyanate derivatization spectrophotometry.Tryptophan were determined by ultraviolet spectrophotometry.The Fisher discriminant analysis was used to analyze the relationship between amino acid and cold or hot nature of traditional Chinese material medica.Result：Form the determinantion of 18 kinds of amino acid contents,it showed that the average amino acid contents of the heat is 1.32 times than the one of the cold,the correct rate is ninety percent analyzed by Fisher discriminant analysis.Conclusion：Form the results of fisher discriminant of 18 variables,it revealed that there are many differences between the cold and hot property in 18 kinds of amino acid contents.Based on the analysis of the determined data,a discrimination function was established and it could distinguish cold and hot property of Chinese herbs.

关 键 词：中药药性 寒热 氨基酸 判别函数 

分 类 号：R284.1[医药卫生—中药学]