灵芝配方颗粒中麦角甾醇的含量测定  

Determination of Ergosterol in Lingzhi Dispensing Granules

作　　者：陈勇[1] 曾萍[1] 温庆伟 胡丰[1] 杨晨[1] 刘瑛丽[1] 巫世红[1] 

机构地区：[1]广西中医学院,南宁530001 [2]培力(南宁)药业有限公司,南宁530007

出　　处：《中国实验方剂学杂志》2010年第12期53-55,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科技攻关项目(桂科攻:0718002-4)

摘　　要：目的:建立灵芝配方颗粒中麦角甾醇的含量测定方法。方法:反相HPLC,流动为甲醇-水(99∶1),检测波长为281 nm,流速1.2 mL·min-1,柱温25℃。结果:麦角甾醇对照品进样量在0.223 7～1.342μg呈良好线性关系,回归方程为Y=1 104.468X+1.168,r=0.999 9,回收率为103.1%,RSD为0.48%(n=6)。结论:本法简便易行,重复性好,可作为灵芝配方颗粒的质量控制方法。Objective： To establish a quantitative method for determination of ergosterol in Lingzhi Dispensing Granule.Method： A RP-HPLC method was used to determine the content of ergosterol with methanol-water（99∶ 1） as the mobile phase and detection wavelength was 281 nm,The flow rate was 1.2 mL.min-1 and the column temperature was 25 ℃.Result： The linearity of ergosterol were good in range of 0.223 7～1.342 μg,and the regression equation was Y=1 104.468 X ＋ 1.168（r=0.999 9）.The average recovery was 103.1% and the RSD value of repeatability was 0.48%（n=6）.Conclusion： This method is simple and reproducible,and it can be used for quality control of Lingzhi Dispensing Granule.

关 键 词：灵芝配方颗粒 麦角甾醇 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]