毛鸡骨草中异夏佛塔苷对照品的制备研究  

Preparation of isoschaftoside reference substance from Abrus mollis

作　　者：宁丽娟[1,2] 叶志文 欧彪 汪豪[1,2] 叶文才[2,4] 赵守训[2] 

机构地区：[1]中国药科大学现代中药教育部重点实验室,江苏南京210009 [2]中国药科大学天然药物化学教研室,江苏南京210009 [3]广西玉林制药有限责任公司,广西玉林537001 [4]暨南大学中药及天然药物研究所,广东广州510632

出　　处：《中草药》2010年第9期1464-1466,共3页Chinese Traditional and Herbal Drugs

基　　金：科技部"十一五"重大新药创制项目(2009ZX09103-315);广西科学研究与技术开发项目(桂科攻0815005-1)

摘　　要：目的建立毛鸡骨草中异夏佛塔苷对照品的制备分离方法。方法取毛鸡骨草药材醇提浸膏的正丁醇萃取部分用硅胶柱色谱分离,氯仿-甲醇（90∶10-70∶30）梯度洗脱,收集异夏佛塔苷富集流份,继续采用低压C18柱色谱及制备HPLC进行分离纯化,通过NMR、MS等波谱方法鉴定化合物结构,经TLC、HPLC-UV及MS联用等技术进行质量分数检测。结果从毛鸡骨草中制备分离的异夏佛塔苷对照品,质量分数〉99%。结论本法得到的异夏佛塔苷对照品符合中药化学对照品的相关技术要求,为药材毛鸡骨草和含毛鸡骨草的成方制剂的质量控制及药效物质基础研究提供化学对照品。Objective To establish a preparative method for reference substance of isoschaftoside from Abrus mollis.Methods The n-butanol fraction from the ethanol extract of A.mollis was subject to column chromatography on silica gel eluted with a gradient of CHCl3-MeOH（90∶10 to 70∶30）.The collected fraction of isoschaftoside was successively purified by low-pressure ODS column and preparative HPLC.Its structure was identified by spectroscopic analysis and the purity was detected by TLC and HPLC-UV-MS.Results The TLC and HPLC analysis showed that the purity of the reference substance was more than 99%.Conclusion The reference substance of isoschaftoside acquired by this method could meet the relative demands of the chemical reference substance in Chinese materia medica.It could be used as a reference standard of the quality control and the research of herbal medicine.

关 键 词：毛鸡骨草 异夏佛塔苷 对照品 制备高效液相色谱 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]