丹参饮对2型糖尿病性心肌病大鼠心肌的保护作用  

Effect of Danshen Decoction on the on the Rats of Type 2 Diabetic Cardiomyopathy

作　　者：倪青[1] 王阶[1] 赵安斌[2] 于斌[2] 王敏[2] 黄春荣[2] 李恩庆[2] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]暨南大学医学院,广东广州510632

出　　处：《中国中医基础医学杂志》2010年第8期680-684,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：中国博士后科学基金一等资助金资助项目(20070410129);中国博士后科学基金第一批特别资助金资助项目(200801166);北京市科委重大资助项目(H020920010330);科技计划资助项目(D08050703020802)

摘　　要：目的:探讨丹参饮对糖尿病性心肌病(DCM)大鼠的保护作用。方法:SD大鼠42只高脂高热量饮食诱导胰岛素抵抗,加单次中等剂量(50mg/kg)腹腔注射链脲佐菌素建立DCM动物模型,12周后采用心电图评价心肌受损,取血检测生化指标,Masson染色检测左室心肌胶原含量,Tunel凋亡试剂盒检测心肌细胞凋亡程度,电镜观察心肌亚细胞结构破坏程度,同时采用免疫组织化学染色检测TSP-1、TGF-β1、Chymase和TRB-3蛋白的表达水平,采用Western blotting技术检测TSP-1、A-TGFβ1和L-TGF-β1蛋白的表达水平变化。实时荧光定量PCR检测TSP-1和TRB-3 mRNA表达水平的变化。结果:与模型组相比,丹参饮组大鼠血糖、胆固醇、甘油三酯明显降低,心肌组织破坏程度较轻,胶原纤维含量显著减少,电镜观察心肌细胞亚细胞结构破坏程度较轻,免疫组化检测心肌TSP-1、TGF-β1以及TRB-3的表达水平、Western检测心肌tsp-1、A-TGFβ1和L-TGF-β1蛋白表达水平均降低,PCR检测TSP-1 mRNA和TRB-3 mRNA表达水平降低。结论:丹参饮可通过多条途径抑制糖尿病心肌病大鼠的心肌纤维化,显著延缓高血糖大鼠糖尿病心肌病的发生进程。Objective： Observe the effect of the DanShen Decoction on the diabetic cardiomyopathy（DCM）.Methods： The rat model of DCM was established by eating a high-fat diet together with injection of high dose streptozotocin（50mg/kg） intraperitoneally.After 12 weeks,electrocardiogram was used to evaluate the damage of the myocardial,blood serum GLU,cholesterin and TG were tested by Clinical Chemistry Test Systems.The content of collagen was quantified by Masson staining.Level of cardiomyocyte apoptosis tested by Tunel apoptosis kit.The mRNA level of TSP-1 and TRB-3 was determined by quantification real time RT-PCR,while the protein level of TSP-1、A-TGF-β1 and L-TGF-β1 was analyzed by Western blotting and the protein level of TSP-1、TGF-β1、TRB-3 and Chymase was analyzed by immunohistochemistry.Results： Compared with control group,the rats blood glucose,cholesterol,triglycerides of DanShen Decoction group were significantly decreased.The Myocardial tissue was less damaged and the collagen content was less than control group.The expression of tsp-1,A-TGF-1 and L-TGF-β1 of DanShen Decoction group in the diabetic cardiac muscle were much less than that in control group.The mRNA level of tsp-1 and TRB-3 were lower than control group by PCR detection.Conclusion： DanShen Decoction can inhibit the process of fibrosis in diabetic cardiomyopathy myocardial rats muscle and delay the formation of diabetic cardiomyopathy through of hyperglycemia rats through many signal pathways.

关 键 词：丹参饮 糖尿病心肌病 凋亡 胶原纤维 TSP-1 TGF-β1 TRB-3 CHYMASE 

分 类 号：R285.5[医药卫生—中药学]