普罗布考对动脉粥样硬化兔肝脏PPARα表达的影响  

Effects of Probucol on PPARα Expression in Liver of Atherosclerosis Rabbits

作　　者：杨文军[1] 费书柯[1] 贺红杰[1] 

机构地区：[1]南华大学附属第二医院普外科,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2010年第6期373-376,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨普罗布考对动脉粥样硬化模型兔肝脏的保护作用及其对过氧化体增殖物激活型受体α表达的影响。方法 16只新西兰大白兔给予高脂饲养8周后,随机平分为2组:高脂组饲以高脂饲料及1%淀粉;普罗布考组在饲以高脂饮食基础上每天给予0.25g普罗布考;另选8只兔为正常对照组给予普通饮食。14周末,处死所有动物,检测血脂、主动脉斑块面积、肝组织病理学等指标,并用RT-PCR和westernblot检测各组肝脏过氧化体增殖物激活型受体αmRNA和蛋白的表达量。结果与高脂组相比,普罗布考组动脉粥样斑块减少,肝脏脂肪病变明显减轻,血清总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇显著降低。高脂组肝脏过氧化体增殖物激活型受体αmRNA和蛋白的表达量明显低于对照组,而普罗布考可上调过氧化体增殖物激活型受体αmRNA和蛋白的表达量。结论普罗布考降低动脉粥样硬化模型兔血浆胆固醇水平,上调其肝细胞过氧化体增殖物激活型受体α表达水平,对动脉粥样硬化模型兔脂肪肝具有治疗作用。Aim To explore the effects of probucol on liver protection and PPARα expression of atherosclerosis rabbits model. Methods 16 male New Zealand white rabbits were fed with 1% cholesterol, 5% lard diet for 8 weeks, and then were randomly divided into two groups ： high-fat group ：maintained cholesterol diet for 6 weeks ; probucol group ： the same cholesterol diet mixed with 0.25 g/d probucol for 6 weeks. Control group was fed with normal diet for 14 weeks. This study detected the change of serum lipid profile, atherosclerotic plaque areas of aortas, histopathology and PPARα mRNA and protein expression of the livers at the end of the study. Results After 6 weeks treatment of probu- col, the levels of total cholesterol （ TC ）, high density lipoprotein cholesterol （ HDLC ）, and low density lipoprotein choles- terol （ LDLC ） were reduced significantly compared with the high-fat group （ P 〈 0.05 ）. The atherosclerotic plaque area of aortas and the histopathology of fatty liver were ameliorated in probucol group compared with high-fat group. The PPARα mRNA and protein expressions in the fatty livers of high-fat group were downregulated compared with control group, and probucol upregulated PPARα mRNA and protein expressions. Conclusion Probucol decreased the serum choles- terol and upregulated PPARa mRNA and protein expressions, which may inhibit the formation of plaques of aorta and fatty liver in atherosclerosis rabbits model.

关 键 词：普罗布考 过氧化体增殖物激活型受体Α 肝脏 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]