Na^＋-H^＋换泵1在胃癌组织中的表达及其临床意义  

Expression of Na^＋-H^＋ exchanger 1 in human gastric carcinoma tissue and its clinical significance

作　　者：牛嫣阳[1] 余佩武[1] 唐波[1] 石彦[1] 郝迎学[1] 

机构地区：[1]第三军医大学西南医院全军普通外科中心,重庆400038

出　　处：《中华胃肠外科杂志》2010年第8期604-607,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金（30972875）;重庆市科技攻关计划项目（CSTC2009AB5035）

摘　　要：目的 探讨胃癌组织中Na^＋-H^＋交换泵1（NHE1）的表达水平及其与临床病理资料的关系.方法 分别应用RT-PCR和Western blot方法检测60例胃癌组织及其相匹配的癌旁胃黏膜组织中NHE1 mRNA及蛋白的表达情况,并进一步分析其表达与胃癌临床病理资料的关系.结果 本组60例患者胃癌组织中NHE1 mRNA及蛋白表达的相对表达量分别为0.786±0.291和1.442±0.175,高于癌旁组织的0.369±0.052和0.348±0.029,差异均有统计学意义（均P〈0.01）.胃癌组织中NHE1 mRNA表达和NHE1蛋白表达显著相关（r=0.264,P〈0.05）.NHE1 mRNA和蛋白表达与肿瘤浸润深度、淋巴结转移及TNM分期有关（均P〈0.05）,而与年龄、性别及分化程度无关（P〉0.05）.结论 胃癌组织中的NHE1 mRNA及蛋白的表达水平均显著升高,可能参与了胃癌的发生发展进程.Objective To determine the expression of Na^＋-H^＋ exchanger 1（NHE1）in human gastric carcinoma tissue and to investigate the association between NHE1 expression and clinicopathological characteristics.Methods The expressions of NHE1 mRNA and protein were detected in both gastric carcinoma tissue（n=60）and adjacent gastric mucosa tissue （n=30）by reverse transcription polymerase chain reaction（RT-PCR）and Western blot.The association between the expression and the clinicopathological characteristics was analyzed.Results The relative expression levels of NHE1 mRNA and protein in gastric carcinoma tissue were 0.786±0.291 and 1.442±0.175.which were significantly higher than those in adjacent gastric mucosa tissue（0.369±0.052 and 0.348±0.029）（P〈0.01）.The expression of NHE1 mRNA was positively correlated with NHE 1 protein in the gastric carcinoma tissue （r=0.264,P〈0.05）.The expressions of NHE1 mRNA and protein were associated with the depth of invasion,lymph node metastasis,and TNM staging（P〈0.05）.However,no statistical difference was found in age,gender,and tumor differentiation（P〉0.05）.Conclusion The expression levels of NHE1 mRNA and protein are significantly up-regulated in gastric carcinoma tissue,which may be involved in the development of gastric carcinoma.

关 键 词：胃肿瘤 Na^+-H^+交换泵 聚合酶链反应 蛋白质印迹法 

分 类 号：R735[医药卫生—肿瘤]