Ad5F35-LMP2重组腺病毒修饰DC体内特异性抗瘤作用的研究  

Tumor suppression in vivo of DC vaccine modified by recombinant adenovirus Ad5F35-LMP2

作　　者：莫武宁[1] 王湛[2] 周玲[2] 詹少兵[2] 唐安洲[1] 黄光武[1] 曾毅[2] 

机构地区：[1]广西医科大学第一附属医院,南宁530000 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室

出　　处：《中华实验和临床病毒学杂志》2010年第4期270-272,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家“863”资助项目（2006AA02A229）;广西科学基金（桂科自0640114）

摘　　要：目的 了解含有EB病毒潜伏膜蛋白2的非复制型重组腺病毒（Ad5F35-LMP2）修饰的DC能否在体内抑制肿瘤细胞生长.方法 人外周血单个核细胞在细胞因子IL-4、GM-CSF和TNF-a诱导下生成树突状细胞.将人的外周血单个核细胞移植入实验组SCID小鼠体内2次,再分别经两次DC与Ad5F35-LMP2-DC免疫SCID小鼠,然后用1×10^6的CNE-2肿瘤细胞接种SCID小鼠皮下,观察肿瘤生长情况.结果 Ad5F35-LMP2-DC组肿瘤的潜伏期明显延长,对照组和DC组小鼠1周左右就出现了肿瘤,而Ad5F35-LMP2-DC组则直到2周左右才出现肿瘤;且肿瘤体积最小,重量最轻,与DC组比Ad5F35-LMP2-DC疫苗组的肿瘤抑制率为64.75%.结论 Ad5F35-LMP2重组腺病毒疫苗修饰的DC在体内可以有效的抑制NPC肿瘤细胞CNE-2生长.Objective To observe whether dendritic cells（DCs） transfected with recombinant adenovirus Ad5F35-LMP2 suppress the proliferation of CNE-2 in vivo. Methods Dendritic cells have been generated with the induction of IL-4,GM-CSF and TNF-a in vitro. After human PBMCs were transplant into SCID mice,SCID mice were vaccinated with Ad5F35-LMP2-DC or untransfected DC. And four days latter,CNE-2 （1×10^6cell for each mouse） inoculated into SCID mouse subcutaneously. The tumor growth was monitored every week. Results The tumor latent period of Ad5F35-LMP2-DC group is longer among three groups. Untransfected DC group and CNE-2 positive control group could be found tumor about one week, but Ad5F35-LMP2-DC group was about two weeks. And the tumor was smaller and lighter than untransfected DC group and CNE-2 positive control group. Average tumor control rate reached 64.75% compared with untransfected DC group. Conclusion Ad5F35-LMP2-DC could effectively suppress the proliferation of CNE2 in vivo.

关 键 词：腺病毒科 疱疹病毒4型 人 病毒包膜蛋白质类 树突状细胞 肿瘤抑制蛋 白质类 

分 类 号：R730.2[医药卫生—肿瘤]