HDAC1和HDAC6蛋白过表达在卵巢浆液性癌中的临床病理学意义  

Over expression of histone deacetylases and their clinicopathological significance in ovarian serous carcinoma

作　　者：韩艳[1] 张美花[1] 金铁峰[1] 张爽[1] 玄延花[1] 林贞花[1,2] 

机构地区：[1]延边大学医学部基础医学院病理学教研部,延吉133000 [2]美国约翰.霍普金斯大学医学院病理系

出　　处：《临床与实验病理学杂志》2010年第4期394-396,401,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(30960120);新世纪优秀人才基金(NCET-07-0734);吉林省科技厅社会发展重点项目(20090442)

摘　　要：目的探讨组蛋白去乙酰化酶(histone deacetylase,HDAC)1和6蛋白表达检测在卵巢浆液性癌中的临床病理学意义。方法选取147例卵巢病变的存档蜡块标本,其中包括39例卵巢浆液性囊腺瘤26例卵巢浆液性交界性囊腺瘤和82例卵巢浆液性囊腺癌。应用免疫组化EnVision法检测HDAC1和HDAC6基因蛋白在上述卵巢病变组织中的表达水平,并分析其蛋白检测的临床病理学意义。结果 HDAC1和HDAC6蛋白分别为胞核和胞质染色,HDAC1蛋白强阳性表达率在卵巢浆液性囊腺瘤、交界性浆液性肿瘤和浆液性囊腺癌中分别为7.7%(3/39)、65.4%(17/26)和80.5%(66/82);HDAC6蛋白的强阳性表达在39例卵巢浆液性囊腺瘤中完全阴性,而在交界性浆液性肿瘤中为34.6%(9/26)、在浆液性囊腺癌中则高达86.6%(71/86),差异均具有统计学意义。结论 HDAC1和HDAC6蛋白在卵巢癌发生发展过程中起重要作用,对于卵巢浆液性癌的诊断具有重要的辅助意义,有望成为卵巢癌治疗的新靶点。Purpose To investigate the clinicopathological significance of histone deacetylases （HDACs） expression in ovarian serous tumors. Methods Total 147 cases of paraffin embedded ovarian lesion blocks were collected,including 39 cases of serous cystadenoma,26 serous borderline cystadenoma,and 82 serous adenocarcinoma. The protein expression of HDAC1 and HDAC6 was detected by immunohistochemistry,and the clinicopathological significance of HDAC1 and HDAC6 expression in ovarian serous carcinoma was analyzed. Results Immunohistochemical staining showed that the strongly positive rate of HDAC1 protein was 7.7% （3/39）,65.4% （17/26）,and 80.5% （66/82） in serous cystadenoma,serous borderline cystadenoma,and serous adenocarcinoma,respectively. Similarly,the HDAC6 protein was negative in all 39 cases of serous cystadenoma,however,the strongly positive rate of HDAC6 protein were 34.6% （9/26） and 86.6% （71/86） in serous borderline cystadenoma and serous adenocarcinomas,respectively. The differences were statistically significant. Conclusions HDAC1 and HDAC6 genes play a key role in the progression of ovarian cancer. The detection of HDAC1 and HDAC6 proteins expression can be used as markers for the early diagnosis of ovarian cancer,and they might be the molecular target for the ovarian cancer therapy.

关 键 词：卵巢肿瘤 组蛋白去乙酰化酶 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]