转化生长因子-β1对绒毛膜癌JEG-3细胞增殖及Smad3、Smad7表达的影响  

Effect of Transforming Growth Factor-β1 on Proliferation and Smad3,Smad7 Protein Expression in JEG-3 Cells

作　　者：许倩[1] 李玉红[1] 李晓茹[1] 张晓红[1] 

机构地区：[1]承德医学院基础医学研究所,067000

出　　处：《天津医药》2010年第9期746-749,共4页Tianjin Medical Journal

基　　金：河北省科技厅课题资助项目(项目编号:07276101D-35);河北省卫生厅课题资助项目(项目编号:05015);河北省教育厅课题资助项目(项目编号:2008303;2009303)

摘　　要：目的:观察转化生长因子(TGF)-β1对绒毛膜癌JEG-3细胞增殖能力及其Smad3、Smad7蛋白表达的影响。方法:体外培养人绒毛膜癌JEG-3细胞,分别给予不同剂量及不同作用时间的TGF-β1进行诱导,四甲基氮唑比色法检测细胞增殖能力变化,蛋白印迹法检测Smad3、Smad7蛋白的表达变化。结果:JEG-3细胞对TGF-β1的诱导作用表现为良好的浓度效应和时间效应。100、200μg/LTGF-β1作用后,细胞增殖率显著增加(P<0.01),Smad3蛋白的相对表达量增加(P<0.05或P<0.01),Smad7蛋白无明显改变(P>0.05);同时100μg/LTGF-β1作用12h后,细胞增殖率显著增加,Smad3蛋白相对表达量增加(P<0.05或P<0.01),Smad7蛋白表达无改变(P>0.05)。结论:外源性TGF-β1能够促进绒毛膜癌JEG-3细胞的增殖,具有良好的浓度效应和时间效应;绒毛膜癌JEG-3细胞逃逸TGF-β1介导的生长抑制作用,可能是由于TGF-β1/Smads信号通路中的Smad3、Smad7蛋白发生异常而致。Objective： To investigate the effect of transforming growth factor （TGF） -β1 on the proliferation and expression of Smad3, Smad7 protein in JEG-3 cells. Methods： The human choriocarcinoma cell line JEG-3 cultured in vitro was given different concentrations and different action times of TGF-β1. The proliferation of JEG-3 cells was measured by MTT assay. The expression of Smad3, Smad7 proteins was measured by Western blotting. Results： JEG-3 cells showed the excellent concentration and time effect responded to the external TGF-β1. The JEG-3 cell proliferation was enhanced after TGF-β1 induction with 100 and 200 μg/L respectively （P 0.01）, meanwhile, The expression of Smad3 protein was increased significantly（P 0.05 or P 0.01）, but the expression of Smad7 protein showed no change（P 0.05）. At the same time, JEG-3 cell proliferation was significantly increased after 12 h 100 μg/L TGF-β1 induction （P 0.05 or P 0.01）. The expression of Smad3 protein was increased significantly（P 0.05 or P 0.01）, but Smad7 protein expression showed no difference（P 0.05）. Conclusion： The external TGF-β1 can stimulate the proliferation of JEG-3 cells, and showing good concentration and time effect. Smad7 protein showed lack of expression and Smad7 protein was expressed at abnormally high levels, which suggested that JEG-3 cells escaped TGF -β1 -mediated growth inhibition may be due to Smad3, Smad7 protein of TGF -β1/Smads pathway.

关 键 词：转化生长因子β1 绒毛膜癌 细胞增殖 Smad3蛋白质 Smad7蛋白质 印迹法 蛋白质 

分 类 号：R575.2[医药卫生—消化系统]