小细胞肺癌PGRP基因的克隆及疫苗构建  

Cloning of PGRP gene from SCLC and construction of DNA vaccine against PGRP

作　　者：周小林[1] 蘧艳峰[1] 薛振伟[1] 谷娟娟[1] 崔梅萍[1] 

机构地区：[1]中国辐射防护研究院,太原030006

出　　处：《免疫学杂志》2010年第9期799-803,共5页Immunological Journal

基　　金：中国辐射防护研究院院设基金项目(A0120080527-08)资助

摘　　要：目的构建重组质粒pcDNA3.1/PGRP,肌肉注射免疫Balb/c小鼠,探讨PGRP的基因免疫效果。方法胶回收纯化的PGRP基因片段及pcDNA3.1质粒经HindⅢ、BamHⅠ双酶切,T4DNA Ligase连接构建成重组质粒pcDNA3.1/PGRP,用PCR方法对其进行鉴定,质粒抽提后进行序列分析;于小鼠后腿双侧肌注免疫,检测各项免疫指标。结果构建的重组质粒经PCR鉴定和测序证明PGRP插入方向正确,免疫检测结果显示重组质粒激活了PGRP特异的CTL和Th1细胞,同时PGRP特异性抗体效价小幅升高。结论构建的重组质粒pcDNA3.1/PGRP具有诱导特异性体液免疫应答和细胞免疫应答的能力,可作为潜在的小细胞肺癌新型疫苗,有进一步研究的意义。We aim to construct the recombinant plasmid pcDNA3.1/PGRP and explore its protective effect on small cell lung cancer （SCLC） in Balb/c mouse. PGRP was inserted into pcDNA3.1 by T4 DNA ligase after PGRP and pcDNA3.1 were digested by restriction endonuclease,then the recombinant plasmid pcDNA3.1/PGRP was identified by PCR amplifying and DNA sequencing. The recombinant plasmid was used to immunize Balb/c mouse by muscle injection. PCR amplifying and DNA sequencing confirmed that the constructed recombinant plasmid contains the exact gene sequence of PGRP. The recombinant plasmid induced CTL and T-helper 1-dominated immune response in mice,as well as antibody against PGRP. The results indicate that the construc-ed recombinant plasmid pcDNA3.1/PGRP can induce both humoral and cellular immune response. Thus this vaccine could be used as a potential candi-date vaccine for the control of SCLC.

关 键 词：小细胞肺癌 前胃泌素释放肽 DNA疫苗 肿瘤免疫 

分 类 号：R734.2[医药卫生—肿瘤]