自动化制备多巴胺转运蛋白显像剂^(11)C-β-CFT及其在正常新生猪脑内PET/CT显像中的应用  

Automatic preparation of dopamine transporter imaging agent ^(11)C-β-CFT and its application in PET/CT brain imaging of newborn piglets

作　　者：张妍芬[1] 王晓明[1] 曹礼[1] 王晓煜[1] 刘长平[1] 李云涛[1] 辛军[1] 郭启勇[1] 

机构地区：[1]中国医科大学附属盛京医院放射科,辽宁沈阳110004

出　　处：《中国医学影像技术》2010年第8期1409-1412,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30770632)

摘　　要：目的在线自动化制备多巴胺转运蛋白显像剂11C-甲基-N-2β-甲基酯-3β-(4-氟-苯基)托烷(11C-β-CFT),并用以进行新生猪PET/CT脑显像。方法使用GE Tracerlab Fx-pro合成模块自动合成11C-β-CFT。对3只正常新生猪进行11C-β-CFT PET/CT 3D动态显像,观察脑内基底节放射性随时间的变化,并绘制时间-活性曲线。结果在线自动化制备的11C-β-CFT放射化学纯度>98%,比活度>370 MBq/μmol,最终获得11C-β-CFT 20 mCi。PET/CT 3D脑动态显像显示,静脉注射后5 min内即可见11C-β-CFT在大脑皮质、小脑及基底节区域放射性分布较高,脑白质放射性分布较低,左右侧放射性浓聚程度对称;随着时间延长,大脑皮质、小脑的放射性明显减低,而基底节显示清晰。结论在线自动化制备11Cβ--CFT简单方便、速度快、放化纯度高,可用于进行新生猪的脑受体方面的研究。Objective To develop an automatic synthesis method for the on-line preparation of dopamine transporter imaging agent 11C-methyl-N-2-β-carbomethoxy-3-β-（4-fluorophenyl）-tropanel（11C-β-CFT）,and to study the regional brain DAT uptake in healthy newborn piglets using PET/CT.Methods 11C-β-CFT was automatively prepared on-line using TRACERlab FX-pro system of GE company.Dynamic scans were carried out in 3 healthy newborn piglets using PET/CT.The changes of distribution of 11C-β-CFT in brain with time elapse were observed,and the curve of activity-time was drawn accordingly.Results The radiochemical purity of 11C-β-CFT was over 98%,and the specific radioactivity was more than 370 MBq/μmol.The synthesis yield was 20 mCi.After injection,11C-β-CFT accumulation in cortical,basal ganglia,and cerebellum was showed clearly at 5 min.The radioactivity in cortical and cerebellum exhibited decrease with time going.The radioactivity mainly accumulated in basal ganglia areas.Conclusion The automatic on-line preparation method is verified in producing 11C-β-CFT of high radiochemical purity for a short period of time.Some brain receptor research on newborn piglets may be carried out with 11C-β-CFT PET/CT imaging.

关 键 词：多巴胺膜转运蛋白 缺氧缺血 脑 正电子发射型体层摄影术 模型 动物 

分 类 号：R-332[医药卫生] R817.4