不同血瘀造模对B_(16)荷瘤鼠肝转移模型的影响  

Effect of Different Methods of Making Models of Blood Stasis Syndrome on Hepatic Metastasis in Tumor-Bearing Mouse Models

作　　者：潘永明[1] 杨玉伟[1] 刘瑞敏[1] 陈亮[1] 朱科燕[1] 屠珏[1] 何欢[1] 陈民利[1] 

机构地区：[1]浙江中医药大学动物实验研究中心/比较医学研究中心,杭州310053

出　　处：《中国实验动物学报》2010年第4期326-330,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省教育厅科研基金项目(20071093);浙江省卫生高层次创新人才培养工程项目

摘　　要：目的比较不同血瘀造模对B16荷瘤鼠肝转移模型的影响。方法取60只6周龄雌性C57BL/6J小鼠,随机分为正常对照组、单纯荷瘤组、BS1(利血平法)组、BS2(游泳复合高分子右旋糖酐法)组、BS3(寒凝复合肾上腺素法)组和BS4(地塞米松复合肾上腺素法)组,首先根据中医理论用不同造模方法形成血瘀模型,然后经脾接种B16黑色素瘤建立肝转移模型。用激光多普勒观察小鼠耳廓微循环变化,并在接种后第15天小鼠眼眶取血,检测红细胞免疫功能和血清唾液酸酶(SA)含量的变化,并麻醉处死动物,观察肝转移情况。结果①单纯荷瘤组和各血瘀荷瘤组小鼠的耳廓微循环血流均明显减少(P<0.01),以BS2组降低显著。②单纯荷瘤组和各血瘀荷瘤组小鼠的红细胞免疫功能亦明显降低(P<0.01),血清SA含量显著升高(P<0.05,P<0.01),以BS2和BS3组变化明显。③肝转移发生率:单纯荷瘤组、BS2组和BS3组均为100%,BS1组为80%,BS4组为60%,肝转移抑制率:BS2组、BS3组、BS1组和BS4组分别为-71.76%、3.90%、16.42%、100.6%。④荷瘤鼠肝转移的肝重与其耳廓微循环血流、红细胞免疫功能和唾液酸酶含量具有显著的相关性(P<0.05,P<0.01)。结论不同血瘀造模对B16荷瘤鼠肝转移模型肝转移发生程度具有明显的影响,应注意血瘀模型的选择,且其与红细胞免疫功能低下有关。Objective The aim of this study was to compare the effect of different methods of making models of blood stasis syndrome on hepatic metastasis of tumors. Methods 60 C57BL /6J mice （female,6 weeks old） were divided into six groups equally： normal control group,non stasis group,BS1 （ induced by reserpine） group,BS2 （ induced by swimming and high polymer dextran） group,BS3 （induced by cold coagulation and adrenalin） group,and BS4 （induced by dexamethasone and adrenalin） group. Models of blood stasis syndrome were generated by different methods in the mice and then hepatic metastases were induced in C57BL /6J mice by intrasplenic injection of B16 melanoma cells. The auricular microcirculation was measured by laser Doppler flowmetry. Blood was collected and the changes of serum SA level and erythrocyte immune function were detected at 14 days after tumor cell inoculation. Changes of hepatic metastasis were also observed. Results （1） The blood flow in auricular microcirculation was significantly decreased in the simple tumor-bearing group and other blood stasis groups （P〈0. 01）,especially in the BS2 group. （2） The erythrocyte immune function of mice were significantly lowered in the simple tumor-bearing group and other blood stasis groups （P〈0. 01）,while serum SA contents were remarkably raised （ P〈0. 05,P〈0. 01 ）,especially in the BS2 and BS3 groups. （ 3 ） The hepatic metastasis incidence rate was 100% in simple tumor-bearing group,BS2 and BS3 groups,80% in the BS1 group and 60% in the BS4 group. The hepatic metastasis inhibition rates of the BS2 ,BS3 ,BS1 and BS4 groups were-71. 76% ,3. 90% ,16. 42% and 100. 6% ,respectively. （ 4 ） There was a significant correlation between the weight of liver with tumor metastasis and the auricular microcirculation,red cell immune function,and SA level （P〈0. 05,P〈0. 01）. Conclusion There is an apparent effect of different methods of making models of blood stasis syndrome on the hepatic metastasis in B16 melanoma-bear

关 键 词：血瘀 B16黑色素瘤 肝转移 红细胞免疫功能 小鼠 

分 类 号：R-332[医药卫生]