TLR9在成牙本质细胞中的表达  

Expression of TLR9 in Odontoblast Cells

作　　者：胡楠[1] 何文喜[1] 王玮[1] 李鹏[1] 余擎[1] 

机构地区：[1]第四军医大学口腔医学院牙体牙髓病科,陕西西安710032

出　　处：《口腔医学研究》2010年第4期464-466,共3页Journal of Oral Science Research

基　　金：国家自然科学基金面上项目(编号:30872869)国家自然科学基金面上项目(编号:30772416)

摘　　要：目的:研究成牙本质细胞中TLR9、DSPP基因表达特征及信号转导途径。方法:采用RT-PCR检测TLR9在小鼠牙髓组织中及TLR9、DSPP在成牙本质细胞系中的表达。用CpGODN-A和CpGODN-B刺激细胞,在时间梯度0、3、6、9、12、24h,检测TLR9、DSPP基因的表达特征。结果:TLR9mRNA在小鼠牙髓组织中有表达。TLR9、DSPPmRNA在成牙本质细胞中都有显著的表达,在CpGODN刺激下显著上调,在6h处于峰值。结论:TLR9在成牙本质细胞中有表达;TLR9的特异性表达对DSPP基因表达量有影响。Objective：To study the gene expression and signal transduction pathways of Toll-like receptors9（TLR9）and dentin sialophosprotein（DSPP）in OLC odontoblast-like cells.Methods：The expression of TLR9in mice pulp tissue and TLR9,DSPP in OLC odontoblast-like cells were detected by semi-quantitative RT-PCR.We used CpG ODN-A and CpG ODN-B to stimulate OLC odontoblast-like cells at different time points of 0,3,6,9,12,24h,and then detected the gene expressions of TLR9and DSPP.Results：The expressions of TLRs mRNA were observed in OLC odontoblast-like cells.Both TLR9and DSPP mRNA had a remarkable expression in OLC odontoblast-like cells with the stimulation of CpG ODN,and the expression peaked at 6h.Conclusion：The results showed that the expression of TLR9in OLC odontoblast-like cells was observed.The specific expression of TLR9had an effect on the expression of DSPP gene.

关 键 词：Toll样受体9(TLR9) 牙本质涎磷蛋白(DSPP) 成牙本质细胞 

分 类 号：R780.2[医药卫生—口腔医学]