PEP-1-SOD1预处理对脑梗死小鼠顶叶皮质的保护作用  

Protective effect of PEP-I-SOD1 pretreatment on parietal cortex of mice with cerebral infarction

作　　者：董敏[1] 席刚明[2] 吴文春[1] 曹雅军[1] 李红云[1] 

机构地区：[1]湖北省襄樊市中医院麻醉科,441000 [2]郧阳医学院附属人民医院神经内科,十堰442000

出　　处：《中华神经医学杂志》2010年第9期897-900,共4页Chinese Journal of Neuromedicine

基　　金：基金项目：湖北省高等学校科学研究青年发展项目（988042）

摘　　要：目的 探讨PEP-1-铜,锌超氧化物歧化酶（PEP-1-SOD1）预处理对小鼠顶叶皮质神经元的保护作用.方法 将健康成年昆明小鼠按随机数字表法分为假手术组、模型组、PEP-1-SOD1组,每组各15只.后两组建立永久性大脑中动脉闭塞模型.造模前30min假手术组及模型组腹腔注射生理盐水200 μL,PEP-1-SOD1组注射PEP-1-SOD1 200 μg.24 h后取各组小鼠顶叶皮质,TTC染色测定梗死灶体积,TUNEL染色测定细胞凋亡,黄嘌呤氧化酶法测定SOD1活性,TBA法测定丙二醛（MDA）含量.结果 与模型组相比,PEP-1-SOD1组梗死灶体积明显缩小,凋亡细胞明显减少,SOD1活性明显升高,MDA含量明显下降,比较差异均有统计学意义（P＜0.05）.结论 PEP-1-SOD1预处理可有效减轻脑梗死后顶叶皮质缺血损伤.Objective To investigate the neuropretective effect of PEP-1-SOD1 pretreatment on the parietal cortex of mice with cerebral infarction. Methods Healthy Kunming-mice were assigned randomly into sham-operated group, model group and PEP-1-SOD1 precondition group （n=15）. And the models of permanent middle cerebral artery occlusion （pMCAO） were established in the later 2 groups.The mice in the sham-operated group and model group were intraperitoneally injected with 200-ul saline and the mice in the PEP-1-SOD1 precondition group were intraperitoneally injected with 200-ug PEP-1-SOD1 fusion protein 30 min before the model inducement, respectively. The parietal cortex was dissected 24 h after the success of model making. Triphenyltetrazolium chloride （TTC） staining was employed to detect the volume of infarction and TUNEL staining was used to detect the cell apoptosis;the content of malondialdehyde （MDA） was measured using the thiobarbituric acid （TBA） method and the activity of SOD 1 was measured by xanthine oxidase method. Results The volume of infarction in the PEP-1-SOD1 precondition group was obviously smaller than that in the model group （P〈0.05）.Compared with those in the model group, significantly reduced apoptotic neural cells were noted in the PEP-1-SOD1 precondition group （P〈0.05）. Compared with model group, increased activity of SOD1 and decreased level of MDA were found in the cell apoptosis （P〈0.05）. Conclusion Precondition with PEP-1-SOD1 fusion protein can efficiently protect the parietal cortex of mice with cerebral infarction.

关 键 词：PEP-1 铜 锌超氧化物歧化酶 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]