U-251人星形细胞瘤系的体外热化疗试验  

Hyperthermia and/or chemotherapy on U-251 human astrocytoma cell line in vitro

作　　者：施巍[1] 郭智霖[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院神经外科,上海200011

出　　处：《中华神经医学杂志》2010年第9期909-912,共4页Chinese Journal of Neuromedicine

摘　　要：目的 探讨热疗、化疗及两者序贯治疗对脑胶质瘤细胞增殖的影响.方法 体外传代培养的人星形细胞瘤系U-251细胞分别通过42℃、44℃高热环境和化疗药物顺铂、鬼臼噻吩甙在37℃恒温箱中孵育1 h;另取星形细胞瘤系U-251细胞同时行热疗和化疗联合应用1 h;再取星形细胞瘤系U-251细胞行序贯治疗,即先用1种处理方法,然后间隔1 h、4h,间隔期间在37℃恒温箱中孵育.治疗后采用MTT法测定肿瘤细胞增殖情况并进行统计学分析.结果 不同温度的热疗和不同种类的化疗药物对肿瘤细胞均可产生有效的杀伤作用,细胞增殖率明显下降.热化疗联合应用较单独应用能明显增加其抗肿瘤的效果,序贯治疗中可见44℃热疗＋化疗组、44 ℃热疗后间隔1 h再行化疗组,其抗肿瘤的效果明显强于其他序贯处理组,差异有统计学意义（P＜0.05）.结论 热化疗能有效杀伤肿瘤细胞,联合应用具有协同效应.两种治疗措施序贯治疗可见44 ℃高热预处理能提高肿瘤细胞对化疗药物的敏感性,但化疗药物的预处理效果相对较差,故该协同效应的机制可能是由于高热损伤了肿瘤细胞膜结构从而增加了药物的穿透所致.Objective To observe the influence of hyperthermia and/or chemotherapy on the cell proliferation of U-251 human astrocytoma cell line. Methods Some cells incubated in vitro were treated by hyperthermia （at a temperature of 42 ℃ and 44 ℃ for 1 h） and chemotherapy （with cis-platinum and teniposide at a temperature of 37 ℃ for 1 h）, respectively; some cells were performed hyperthermia and chemotherapy simultaneously for 1 h; some cells were performed sequential therapy （with hyperthermia or chemotherapy first, and then with the other one 1 and 4 h after the former one at a temperature of 37 ℃）. The cell proliferation was measured by MTT method and statistical analysis was performed. Results Hyperthermia or chemotherapy had cell-killing effects; hyperthermia at different temperatures or chemotherapy with cisplatin or teniposide could obviously decrease the cell proliferation rate. Simultaneous hyperthermia and chemotherapy could increase the anti-tumor effects as compared with hyperthermia or chemotherapy alone. The 2 kinds of sequential therapies （[performing hyperthermia at a temperature of 44 ℃ and chemotherapy 1 h after that at a temperature of 37 ℃ ], [performing hyperthermia at a temperature of 44 ℃ and chemotherapy at the same time]） had the strongest anti-tumor effects among all the kinds of sequential therapy, simultaneous hyperthermia chemotherapy have synergistic effects（P〈0.05）. Conclusion Hyperthermia and/or chemotherapy have cell-killing effects;hyperthermia at a temperature of 44 ℃ can increase the susceptibility of chemotherapy, possibly resulting from destroying of cytomembrane and thus increasing the penetrability of cisplatin or teniposide.

关 键 词：星形细胞瘤 抗肿瘤联合化疗方案 顺铂 鬼臼噻吩甙 热疗 细胞增殖 

分 类 号：R739.4[医药卫生—肿瘤]