反义核酸抑制miR-20、miR-106表达对293T细胞增殖的影响  

Inhibition of 293T cell proliferation by blocking miR-20 and miR-106 expression with antisense RNA

作　　者：李有杰[1,2] 张帅[2] 高宗华[2] 张文娟 谢书阳[2] 张鹏举[1] 姜安丽[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012 [2]滨州医学院医学分子遗传研究所,山东烟台264003 [3]烟台山医院血液科,山东烟台264000

出　　处：《中国病理生理杂志》2010年第9期1665-1669,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30801324;No30900550);山东省自然科学基金资助项目(NoZR2009CQ033);烟台市科学技术发展计划资助项目(No2008162)

摘　　要：目的:通过反义核酸技术抑制miR-20、miR-106的表达,检测对293T细胞增殖的影响。方法:应用特异性针对miR-20、miR-106的反义核酸转染293T细胞,用形态学、流式细胞技术研究反义核酸对细胞增殖的抑制作用,用定量PCR和ELISA研究miRNA和相关靶基因的表达。结果:研究发现反义核酸可有效抑制miR-20和miR-106的表达,并明显抑制293T细胞的增殖;研究还表明反义miR-106可明显上调抑癌基因Rb的表达。结论:反义核酸通过抑制miRNA表达,上调抑癌基因Rb的表达,能明显抑制293T细胞增殖。AIM： To inhibit 293T cell proliferation by reducing miR-20 and miR-106 expression with antisense RNA. METHODS： Antisense RNA （ or antisense oligonucleotides,ASO） specific to miR-20 and miR-106 was synthesized,and the 293T cells were treated with these antisense RNA. Then,the cell proliferation derived from suppression of antisense RNA was studied by microscopy and fluorescence-activated cell sorter. The expression of miRNA and its target gene Rb were analyzed by real-time PCR and ELISA,respectively. RESULTS： Our results showed that ASO could inhibit the expression of miR-20 and miR-106 effectively and inhibit 293T cell proliferation. The results also demonstrated that ASO specific to miR-106 could up-regulate anti-oncogene Rb expression. CONCLUSION： ASO could inhibit 293T cell proliferation by inhibiting miRNA expression and up-regulating Rb expression.

关 键 词：RNA 反义 MIRNA 293T细胞 细胞增殖 基因表达 

分 类 号：R394.3[医药卫生—医学遗传学]