苦参碱对淋巴瘤Raji细胞的增殖及survivin蛋白表达的影响  

Effect of matrine on the proliferation of lymphoma Raji cells and survivin expression

作　　者：唐友红[1] 钟美佐[1] 余兴[2] 刘巍[1] 黄进[1] 

机构地区：[1]中南大学湘雅医院肿瘤科,湖南长沙410008 [2]广州市红十字会医院,广东广州510000

出　　处：《现代肿瘤医学》2010年第9期1687-1691,共5页Journal of Modern Oncology

摘　　要：目的:研究苦参碱对淋巴瘤Raji细胞的生长抑制作用,并探讨其可能的作用机制。方法:MTT比色法测定细胞增殖抑制率;Hoechst33342荧光染色及电镜观察细胞形态及结构变化;免疫细胞化学染色法观察survivin蛋白表达的改变。结果:一定浓度苦参碱作用Raji细胞后,细胞增殖受到明显抑制,且抑制率呈浓度和时间依赖性;荧光显微镜下可见核浓缩,聚集,碎裂等典型的凋亡特征;电镜下可见到凋亡小体形成;免疫细胞化学染色法显示随着苦参碱作用浓度增加,survivin蛋白表达降低。结论:苦参碱在一定浓度范围内可以明显抑制恶性淋巴瘤Raji细胞增殖,并下调抗凋亡基因survivin的表达。Objective：To investigate the growth inhibiting effects and the possible mechanism of matrine on Raji cells. Methods： Human Burkitt＇s lymphoma cell Raji was co-cultured with matrine in different concentrations.MTT assay was used to measure the level of the proliferation of Raji cells; the morphological changes of cells were observed by fluorescence microscope and transmission electron microscope, the expression of survivin was measured with immunocytochemistry and image analysis.Results：In certain range of concentration,matrine could obviously refrain proliferation of Raji cells in a dose and time dependent manner; In result of fluorescence microscope and TEM,microvillus disappeared,chromatin condensed to high electron density,nucleus broken to pieces and large deciduous apoptosis bodies appeared after being treated with matrine.The protein level of survivin was down regulated as the concentration of matrine increased.Conclusion：In certain range of concentration,matrine could obviously inhibit the proliferation of human Burkitt＇s lymphoma Raji cells.The expression of survivin was down regulated by matrine.

关 键 词：苦参碱 RAJI细胞 细胞凋亡 SURVIVIN 

分 类 号：R730.53[医药卫生—肿瘤] R733.1[医药卫生—临床医学]