MAGE融合疫苗的构建与表达  

Construction and expression of MAGE combinative vaccine

作　　者：陈连宏[1] 马宁强[1] 王军伟[1] 张志培[1] 

机构地区：[1]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2010年第9期1705-1707,共3页Journal of Modern Oncology

摘　　要：目的:构建MAGE1和MAGE3融合表达载体,并获得抗血清。方法:采用分步交叉亚克隆方法将两个不同片段连接于表达载体,分组对照优化表达条件,用SDS-PAGE和Western-blot检测表达与纯化结果,将纯化蛋白免疫动物后用琼脂双扩和ELISA方法检测抗血清效价。结果:成功构建了MAGE1-MAGE3表达载体并获得纯化蛋白;免疫动物后获得高效价的抗血清。结论:得到纯度高,变性度低的融合蛋白。Objective：To construct combinant MAGE1 and MAGE3 expression vector and acquirement of rabbit anti-serum of combinant protein.Methods：To construct expression vector by truncated sub-clone and cross clone; verified by SDS-PAGE and Western-blot; agar precipitation and ELISA detected the titer of anti-serum.Results：Successfully constructed the expression vector on pET-28a.Conclusion：Acquired the optimal expression conditions and combinant protein,and obtained high titer anti-serum.

关 键 词：黑色素瘤抗原 原核表达 肿瘤 

分 类 号：R730.51[医药卫生—肿瘤]