外周血单个核细胞端粒酶hTERT mRNA与葡萄胎恶变的临床研究  

Study on neoclassical of hydatidiform mole by detecting telomerase reverse transcriptase mRNA expression in peripheral blood mononuclear cells

作　　者：鲍立军[1] 李芬[1] 邹余粮[1] 

机构地区：[1]西安交通大学第一附属医院妇产科,陕西西安710061

出　　处：《现代肿瘤医学》2010年第9期1811-1814,共4页Journal of Modern Oncology

基　　金：陕西自然基金资助项目(编号:2005C265)

摘　　要：目的:了解外周血单个核细胞hTERT mRNA在葡萄胎自然转归及恶变中的表达变化,探讨其在判断葡萄胎预后中的应用价值。方法:FQ-RT-PCR法检测葡萄胎患者外周血单个核细胞hTERT mRNA的表达水平;将hTERT与GAPDH拷贝数之比的100倍作为标准化hTERTNhTERT);随访每一例葡萄胎患者,根据预后将其分为试验组8例(恶变组)和对照组22例(自然转归组);比较两组葡萄胎患者外周血单个核细胞hTERT mRNA的表达水平。结果:葡萄胎实验组和对照组外周血单个核细胞hTERT mRNA,分别为6.31±0.32和1.21±0.65,两者比较有显著差异(P<0.01)。结论:FQ-RT-PCR方法能对端粒酶hTERT mRNA进行准确高效的定量检测;外周血单个核细胞端粒酶hTERT mRNA的表达水平可能用于早期判断葡萄胎的预后。Objective：Using real-time fluorescent quantitative RT-PCR assay, to detect human telomerase reverse transcriptase （hTERT） mRNA in hydatidiform mole in peripheral blood mononuclear cells,and to analyze the correlation between the expression level of hTERT mRNA and prognosis of hydatidiform mole ,and to evaluate the clinical value of quantitative determination of hTERT mRNA in the diagnosis of hydatidiform mole. Methods： A real-time fluorescent quantitative RT-PCRassay （fQ RT-PCR） assay based on Taxman fluorescence method and light-cycler system was used to quantify the full range of hTERT mRNA copy numbers in 30 samples of hydatidiform mole and neoplasia of hydatidiform mole. The normalized hTERT （NhTERT） was standardized by quantifying the number of GAPDH transcripts as internal control and expressed as100×（hTERT/GAPDH） ratio. Based on the prognosis of hydatidiform mole,patients were divided into two groups：experimental group and control group,to compare the telomerase reverse transcirptase mRNA expression in peripheral blood mononuclear cells. Results： NhTERT in experimental group and control group were 6.31±0.32 and 1.21±0.65,respectively （P〈0.001）. Conclusion： Quantitative determination of hTERT mRNA by RQ RT-PCR is a rapid and sensitive method,hTERT in peripheral blood mononuclear cells may have potential use as a biomarker for the early detection of the prognosis of hydatidiform mole.

关 键 词：FQ-RT-PCR 葡萄胎 端粒酶 逆转录酶 

分 类 号：R737.33[医药卫生—肿瘤]