牛胰多肽(BPP)及去氧胆酸盐(DOC)与DMPC脂质体相互作用的激光拉曼光谱研究  

RAMAN SPECTRA STUDY ON THE INTERACTION OF DMPC DISPERSION WITH PANCREATIC POLYPEPTIDE (BPP) AND DEOXYCHOLATE (DOC)

作　　者：聂松青[1] 林克椿[1] 杨先春 

机构地区：[1]北京医科大学生物物理教研室 [2]石油化工研究院

出　　处：《生物物理学报》1990年第3期344-348,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金资助

摘　　要：本文报导用激光拉曼光谱技术研究牛胰多酞(BPP)和去氧胆酸盐(DOC)与DMPC脂质体的相互作用.结果表明,BPP与DMPC之间存在较强的疏水作用,从I_(2880cm)^(-1)/I_(2345cm)^(-1)强度比说明,BPP能使磷脂分子间协同作用加强,抑制由DOC所产生的磷脂分子间作用减弱.从C—C(?)动强度比I_(1998cm)^(-1)/I_((?)25cm)^(-1),说明BPP使磷脂分子内部gauche/trans构象比值下降,同时表现出抑制由DOC产生的guache/trans比值升高的作用.此外,BPP与DMPC作用后,磷脂头部基团外C—N伸缩振动波数向低波数向低波数方向从715cm^(-1)移至710cm^(-1),I_(715cm)^(-1)/I_(1295cm)^(-1)强度比值降低,提示BPP与DMPC之间除了有较强的疏水作用外,同间也存在静电相互作用.This paper reports the Raman spectra study on the interaction of BPP and DOC with DMPC liposomes. The results showed: 1, BPP enhanced the cooperative effect of phospholipid molecules and inhibited the action by DOC which weakened the interaction between phospholipids and decreased the orderness by I2880cmI/I2845cm-1 changes. 2, BPP decrease the ratio of Guache/trans induced by DOC from changes of I1098cm-1/I1125cm-1. 3, 715cm-1 band of DMPC shifteP to 710cm-1, i.e. toward the lower wavenumber after adding BPP and I-15cm-1/I1295cm-1 decreased. The above results indicates that there were not only hydrophobic effect but also electrostatic effect of BPP on phospholipids.

关 键 词：BPP DOC DMPC 激光拉曼光谱 

分 类 号：Q631[生物学—生物物理学]