黄体酮对大鼠局灶性脑缺血再灌注后大脑皮质神经细胞凋亡和Caspase-3 mRNA及蛋白表达的影响  

Effect of Progesterone on neuronal apoptosis and expression of caspase-3 mRNA and protein in cerebral cortex after brain ischemia-reperfusion injury in rats

作　　者：李昕[1] 王建平[2] 张博爱[1] 蒋超[2] 刘春岭[2] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052 [2]郑州大学第五附属医院,河南郑州450015

出　　处：《现代中西医结合杂志》2010年第28期3571-3573,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河南省教育厅自然科学研究计划项目(2009A320026)

摘　　要：目的观察黄体酮对大鼠局灶性脑缺血再灌注后神经细胞凋亡与Caspase-3mRNA及蛋白表达的影响。方法用线栓法制作脑缺血再灌注损伤模型。采用PCR和Western blot技术分别对缺血区皮质Caspase-3 mRNA及蛋白表达情况进行测定。TUNEL染色检测神经元的凋亡情况。结果与脑缺血再灌注组和溶剂对照组相比,黄体酮治疗组大鼠脑部Caspase-3 mRNA及蛋白的表达水平明显减少(P均<0.05),神经元的凋亡数量显著下降(P均<0.05)。结论黄体酮能显著减少Caspase-3 mRNA及蛋白表达,抑制神经细胞凋亡,对脑缺血再灌注损伤发挥保护作用。Objective It is to observe the effects of Progesterone on neuronal apoptosis and the expressions of Caspase-3 mRNA and protein in focal cerebral ischemia/reperfusion rats,and to explore the mechanism of brain protection of Progesterone.Methods Rat cerebral ischemia reperfusion injury models were established with suture emboli method.The expression of Caspase-3 mRNA was analyzed by PCR.Western blot technique was applied to detect the expression of Caspase-3 protein.The apoptosis of neuron was detected by TUNEL stain.Results Compared to ischemia-reperfusion injured group and vehicle-injected control group,the expression levels of Caspase-3 mRNA and protein were decreased significantly and the cell numbers of apoptosis also markedly decreased after the administration of Progesterone（P0.05）.Conclusion Progesterone can significantly decrease the expression of Caspase-3 mRNA and protein,inhibit neuronal cell apoptosis so to play a protecting role in cerebral cortex caused by ischemia reperfusion.

关 键 词：黄体酮 脑缺血再灌注 凋亡 CASPASE-3 CASPASE-3MRNA 

分 类 号：R-332[医药卫生]