苦参碱微乳透皮吸收制剂对急性肝损伤小鼠血清ALT、AST水平及肝体指数的影响  

Effects of matrine microemulsion on serum ALT,AST levels and hepatopancreas somatic indices in mouse with acute liver injury

作　　者：吕建华 魏红 刘俊宁 宋小莉[2] 

机构地区：[1]山东医学高等专科学校,济南250002 [2]山东中医药大学

出　　处：《山东医药》2010年第35期12-13,共2页Shandong Medical Journal

基　　金：山东省自然科学基金项目(Y2007C078)

摘　　要：目的观察苦参碱微乳制剂对小鼠四氯化碳(CC l4)肝损伤的保护作用。方法将60只昆明种小鼠随机分为正常对照组、模型组、阳性对照组和苦参碱高、中、低剂量组各10只。阳性对照组及模型组分别予苦参素胶囊内容物水溶液及生理盐水200 mg/kg灌胃,苦参碱高、中、低剂量组分别予苦参碱微乳制剂200、100、50 mg/kg皮肤给药,均为1次/d,连续用药14 d。其后除正常对照组外,余五组均采用CC l4制作急性肝损伤模型,16 h后测定各组血清ALT、AST水平;处死小鼠取出肝脏称重并计算其与体质量的比值(肝体指数)。结果与模型组相比,余五组肝体指数均显著降低(P<0.05或0.01),其中苦参碱高、中剂量组和阳性对照组血清ALT、AST水平亦显著降低(P均<0.05)。结论苦参碱微乳透皮吸收制剂对小鼠急性CC l4肝损伤具有保护作用,且呈一定剂量依赖性。Objective To observe the protective effects of matrine microemulsion on mouse liver injury by carbon tetrachloride（CCl4）.Methods Sixty Kunming mouse were divided into six groups randomly with 10 in each,which including normal control group,model group,matrine microemulsion groups（high,medium and low-dose） and positive control group.The positive control group and model group given 200 mg/kg aqueous solution of oxymatrine and normal saline by intragastric administration respectively,the matrine microemulsion high,medium and low-dose groups by intragastric administration were treated with 200,100,50 mg/kg matrine microemulsion precutaneously,once a day ×14 ds.Then the five groups beside normal control group were made model of liver injury by CCl4,the serum ALT and AST levels were detected 16 hs later;the mosue were put to death and the livers were weighted,hepatopancreas somatic indices were calculated.Results Compared with the model group,the hepatopancreas somatic indices in the other five groups decreased significantly（P0.05 or 0.01）,and the ALT and AST decreased significantly too in the groups of high and medium doses of matrine microemulsion and the positive control group（P0.05）.Conclusion Matrine microemulsion transdermal delivery play an important role in prevention of acute CCl4 liver injury in mouse,and which presents a dose-dependent manner.

关 键 词：苦参碱 微乳制剂 苦参素 小鼠 四氯化碳 肝损伤 

分 类 号：R575.3[医药卫生—消化系统]