醋酸钙不动杆菌PHEA-2苯酚降解调控蛋白MphR化学信号域的研究  

The Study of Signal Receiver Domain of Phenol Regulator in Actnetobacter calcoaceticus PHEA-2

作　　者：彭子欣[1] 于海英[1,2] 战嵛华[1] 陈明[1] 林敏[1] 张维[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081 [2]中国农业大学生物学院,北京100193

出　　处：《生物技术通报》2010年第10期86-92,共7页Biotechnology Bulletin

基　　金：国家自然科学基金(30770076);国家转基因生物新品种培育重大专项课题(2008ZX08012-001)

摘　　要：MphR是醋酸钙不动杆菌PHEA-2调控苯酚代谢的激活蛋白。通过分析苯酚羟化酶启动子的lacZ融合子(PmphK::lacZ)在21种苯酚及苯酚衍生物诱导时的β-半乳糖苷酶活性,确定了调控蛋白MphR化学信号域可以识别苯酚,邻甲酚,间甲酚,邻氯苯酚等酚类衍生物。大肠杆菌中逐段截短MphR化学信号域试验证实,当化学信号域截短后,MphR受苯酚诱导激活转录的功能丧失。结果表明,化学信号域对于苯酚激活转录功能极为重要。MphR is a positive regulator of phenol hydroxylase gene （mphKLMNOP） of Acinetobacter calcoaceticus PHEA-2. Tran- scriptional analysis using PaphK ：lacZ fusions revealed that, of 21 tested compounds, phenol, o-cresol, m-cresol and 2-chlorophenol et at. stimulated the transcription of mphK gene in the presence of mphR gene as effectors. Deletion analysis the A-domain of MphR showed that no activities were detected in any MphR deletions independent of the presence or absence of phenol. The results showed that the A domain of MphR was essential for the transcription of phenol hydroxylase gene.

关 键 词：醋酸钙不动杆菌PHEA-2 MphR 效应物识别谱 Β-半乳糖苷酶活性 

分 类 号：X172[环境科学与工程—环境科学]