革胡子鲶GH成熟肽在大肠杆菌中表达条件的优化  

Optimization of Expression Conditions for Mature Peptide of Growth Hormone from Clarias lazera in Escherichia coli

作　　者：杨学明[1,2] 何荆洲[2] 张立[1] 黄雄军 郭亚芬[2] 蒋和生[2] 

机构地区：[1]广西水产研究所,南宁530021 [2]广西大学动物科技学院,南宁530005 [3]贵港扬翔饲料有限公司,贵港537100

出　　处：《生物技术通报》2010年第10期182-184,共3页Biotechnology Bulletin

基　　金：广西自然科学基金项目(桂科自0542035)

摘　　要：将携带革胡子鲶(Clarias lazera)生长激素(GH)成熟肽cDNA序列的重组表达载体pRSET-mGH转化入大肠杆菌Escherichia.coliBL21(DE3)pLysS中进行融合蛋白的表达。表达条件优化试验表明,表达菌株接种SOB培养基,培养2-3h后可用IPTG进行融合蛋白诱导表达;IPTG最佳诱导浓度为1.5 mmol/L,最佳诱导时间为4 h。本试验构建的原核表达体系为下一步革胡子鲶生长激素促生长制剂的研制奠定了基础。The recombinant vector pRSET-mGH containing cDNA of mature peptide of growth hormone （mGH） from Clarias lazera was successfully expressed in E. coli BL21 （DE3） pLysS. Optimization test for expression condition showed that pRSETA-mGH/ BL2t should be firstly cultured in SOB medium for 2 -3 h at 37~C before induction,then induced with IPTG to express mGH fusion protein. The appropriate IPTG concentration is 1.5 mmol/L, and the suitable inducing time is 4 h. This prokaryotic expression system should provide great probabilities for further research on biotechnological preparations of GH from C. lazera.

关 键 词：革胡子鲶 生长激素 原核表达优化 

分 类 号：S917.4[农业科学—水产科学]