转TrxS基因大麦矮化突变株系种子贮藏蛋白的变化  

Analysis on Seed Storage Protein of Two Barley Dwarf Mutants

作　　者：景晓东[1] 尹钧[1] 牛洪斌[1] 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,河南郑州450002

出　　处：《河南农业科学》2010年第9期9-12,共4页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30370877)

摘　　要：以大麦2个稳定矮化突变株系HS-1和HS-2为材料,对其株高性状、种子贮藏蛋白含量及醇溶蛋白带型等进行了研究。结果表明,矮化株系HS-1和HS-2的平均株高分别为81.30 cm和81.92 cm,显著低于非转基因对照和正常转基因株系（P〈0.05）;胚乳内清蛋白、球蛋白、醇溶蛋白、麦谷蛋白、可溶性总蛋白的含量均低于非转基因对照,其中醇溶蛋白、麦谷蛋白和可溶性总蛋白含量与非转基因对照及个别正常转基因株系间差异达显著水平（P〈0.05）。SDS-PAGE结果显示,矮化株系醇溶蛋白在35.0-45.0 kD区段与非转基因对照存在明显差异,并表现为高分子量聚集体数量减少,低分子量聚集体增加。In the paper,two dwarf mutants of barley（HS-1 and HS-2）,originally identified as two barley（Hordeum vulgare L.） dwarf mutants in transgenic lines produced in our laboratory,were used to analyze the mutant phenotype and the characteristics of seed storage proteins.The results showed that both the two mutants exhibited dwarf phenotype under normal growth conditions,and the mean plant height of HS-1 and HS-2 were 81.30cm and 81.92cm respectively,significantly shorter than that of wild type（P〈0.05）.The four fractions of seed storage protein and the soluble protein in endosperm were extracted from mature seeds of two dwarf mutants（HS-1 and HS-2） and five transgenic lines（Htg-1-6,Htg-2-1-1,Htg-3-1,Htg-4-2-9 and Htg-15-7-19）,and the protein content of each fraction was determined.The results showed that the content of total soluble proteins in mutants significantly lower than that in wild type and several trasngenic lines（P〈0.05）.To further quantify the protein composition,SDS-PAGE were used to analysis the profiles of hordeins and total soluble proteins in mature seeds.The polymorphism was observed in B group hordeins,which molecular weights of peptides ranged from 35.0kD to 45.0kD,while no differences were detected in total proteins.

关 键 词：大麦 矮化突变体 种子贮藏蛋白 SDS-PAGE TRXS基因 

分 类 号：S512.1[农业科学—作物学]