槐角丸中槲皮素和没食子酸含量的HPLC测定  

Determination of the genistein and quercetin in Huaijiao Wan By RP-HPLC

作　　者：刘思曼[1] 边清泉[1] 

机构地区：[1]绵阳师范学院化学与化学工程学院,四川绵阳621000

出　　处：《化学研究与应用》2010年第9期1195-1198,共4页Chemical Research and Application

基　　金：四川省教育厅重点项目(09ZC019)资助;绵阳市科技局自然科学研究重点项目(08Y002-3)资助

摘　　要：Objective:To determine the contents of the four efficacious elements(quercetin;ellagic acid) in Huaijiao Wan by RP-HPLC.Method:High Performance Liquid Chromatograph is employed with a Column of Diamonsil ODS-C18(4.6mm×250mm,5.0μm) and a mobile phase of MeOH-H2O-HAc(2∶3∶0.15),the detection wavelength being 270 nm and the column temperature being 300C.Result:Nice linear relation exists when,quercetin is within 0.2048-1.229μg and ellagic acid within 0.5325-3.192 μg.The R value of each group is 0.9991 and 0.9998.The average rate of recovery of the two groups is between 98.0% and 99.1%.The RSD of each group is 1.02% and 0.40%(n=5).Conclusion:With this method,the two efficacious elements could be determined simultaneously.And for its convenience,nicety,high sensitivity and fidelity,it is applicable to the quality examination of Huaijiao products and to manufacturers’ control of quality.Objective：To determine the contents of the four efficacious elements（quercetin;ellagic acid） in Huaijiao Wan by RP-HPLC.Method：High Performance Liquid Chromatograph is employed with a Column of Diamonsil ODS-C18（4.6mm×250mm,5.0μm） and a mobile phase of MeOH-H2O-HAc（2∶3∶0.15）,the detection wavelength being 270 nm and the column temperature being 300C.Result：Nice linear relation exists when,quercetin is within 0.2048-1.229μg and ellagic acid within 0.5325-3.192 μg.The R value of each group is 0.9991 and 0.9998.The average rate of recovery of the two groups is between 98.0% and 99.1%.The RSD of each group is 1.02% and 0.40%（n=5）.Conclusion：With this method,the two efficacious elements could be determined simultaneously.And for its convenience,nicety,high sensitivity and fidelity,it is applicable to the quality examination of Huaijiao products and to manufacturers＇ control of quality.

关 键 词：高效液色谱法 槐角丸 槲皮素 没食子酸 

分 类 号：O657.72[理学—分析化学]