HPLC-PDA测定银屑灵流膏中5种成分含量  

Simultaneous determination of chlorogenic acid, peoniflorin, isofraxidin, ferulaic acid and astilbin in Yinxieling liquid extract by HPLC-PDA

作　　者：陈有军[1] 赵瑞芝[1] 赵莹[1] 卢传坚[1] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510006

出　　处：《中成药》2010年第9期1522-1525,共4页Chinese Traditional Patent Medicine

基　　金：广东省自然科学基金资助项目(T004847)

摘　　要：目的:建立银屑灵流膏(当归、赤芍、川芎等)中绿原酸、芍药苷、异秦皮啶、阿魏酸和落新妇苷5种成分含量的测定方法,控制产品质量。方法:采用HPLC-PDA法,色谱柱为Kromasil100-5C18,流动相为甲醇-0.1%冰醋酸,梯度洗脱,梯度为0～10min(10∶90～30∶70),10～40min(30∶70～40∶60);流速为1.0mL/min;检测波长绿原酸、异秦皮啶、阿魏酸为320nm,落新妇苷为289nm,芍药苷为230nm。结果:上述条件下5种成分分离良好,绿原酸在2.66～31.86μg/mL(r=0.9999),芍药苷在34.95～419.37μg/mL(r=0.9999);异秦皮啶在0.96～11.55μg/mL(r=0.9999);阿魏酸在0.82～9.84μg/mL(r=0.9999);落新妇苷在6.58～78.92μg/mL(r=0.9999)范围内线性关系良好,5种成分的加样回收率在96.6%～103.7%之间;RSD小于3.83%。结论:该方法方便、准确、灵敏度高,可作为银屑灵流膏中绿原酸、芍药苷、异秦皮啶、阿魏酸和落新妇苷5种成分的质量控制方法。AIM： To establish a quantification method for determining chlorogenic acid,peoniflorin,isofraxidin,ferulaic acid and astilbin in Yinxieling liquid extract（Radix Angelicae sinensis,Radix Paeoniae rubra,Rhizoma chuanxiong,etc. ）,and to control the quality of the preparation. METHODS： High performance liquid chro-matography-photodiode array （HPLC-PDA） method was developed in this study. The column was Kromasil 100-5 C18,the mobile phase consisted of methanol-0. 1% acetic acid（10 ∶ 90-30 ∶ 70） during 0-10 min,and （30 ∶ 70 ～ 40 ∶ 60）during 10-40 min in gradient elution with flow rate at 1. 0 mL/min. The detection wavelength was set at 320 nm for chlorogenic acid,isofraxidin and ferulaic acid,289 nm for astilbin and 230 nm for peoniflorin. RESULTS： The calibration curves of the five components were excellent,the linear ranges of chlorogenic acid,peoniflorin,isofraxidin,ferulaic acid and astilbin were in the ranges of 2. 66-31. 86 μg/mL（r = 0. 999 9）,34. 9-419. 37 μg/mL（r =0.999 9）,0.96-11.55 μg/mL（r =0.999 9）,0.82-9.84 μg/mL（r =0.999 9）,6.58-78.92 μg/mL （r =0.999 9）,respectively. The average recoveries ranged from 96.6% to 103.7%,the relative standard deviations were less than 3. 83% . CONCLUSION： The established method is convenient and accurate with a satisfactory separation and high sensitivity. It is suitable for the quality control of the five components in Yinxieling liquid extract.

关 键 词：HPLC—PDA 银屑灵 绿原酸 芍药苷 异秦皮啶 阿魏酸 落新妇苷 

分 类 号：R927.2[医药卫生—药学]