海水微生物絮凝剂产生菌HY-20筛选及絮凝活性研究  

Isolation and Activity Exploration of Flocculating Active Marine Bacteria HY-20

作　　者：张秀芝[1] 王静[1] 张雨山[1] 杨波[1] 曾兴宇[1] 邱金泉[1] 

机构地区：[1]国家海洋局天津海水淡化与综合利用研究所,天津300192

出　　处：《海洋技术》2010年第3期105-107,127,共4页Ocean Technology

基　　金：国家海洋局青年基金资助项目(2005609);国家"十一五"科技支撑计划资助项目(2006BAB03A08)

摘　　要：从海边污泥中分离得到一株高效絮凝剂产生菌HY-20,经鉴定为假单孢菌。通过碳、氮源及初始pH等培养条件试验研究,获得了该菌株生产絮凝剂的最佳培养条件:最适碳源为葡萄糖,最适氮源为脲+酵母膏;初始pH值为7～8,温度为30℃,摇床转速130 rad/min,培养时间为72 h,絮凝率可达95%;絮凝剂有效絮凝成分主要存在于分泌物中。A high flocculating active strain HY-20 was isolated from coastal sludge, which was identified as Pseudomonas sp. Differ- ent carbon sources, nitrogen sources, initial pH of culture and other incubation conditions were studied to explore the optimum fer- mentation conditions. The optimum conditions for collecting flocculants were： glucose as carbon source, urea plus yeast extract as nitrogen source, initial pH value 7-8, incubation temperature 30℃, shaking speed 130 rad/min, incubation time 72 h, respectively. The flocculation rate could reach up to 95%. The flocculating components were mainly in secretions.

关 键 词：微生物絮凝剂 絮凝活性 培养条件 活性分布 

分 类 号：X172[环境科学与工程—环境科学]