莫诺苷对局灶性脑缺血再灌注大鼠Caspase-3活化程度的影响  

Effects of Morroniside on Activation of Caspase-3 in Rats with Focal Cerebral Ischemia/Reperfusion

作　　者：汪莹[1] 高东明[1] 许栋明[2] 王文[3] 艾厚喜[3] 张丽[3] 李林[3] 

机构地区：[1]辽宁医学院生理教研室,辽宁锦州市121000 [2]科技部火炬高新技术产业开发中心,北京市100038 [3]首都医科大学宣武医院,北京市100053

出　　处：《中国康复理论与实践》2010年第9期801-802,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：科技部“重大新药创制”科技重大专项(2009ZX09103-366);国家自然科学基金项目(30973893);北京市自然科学基金项目(7102077)

摘　　要：目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠海马caspase-3活化程度的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,缺血30min,再灌注72h。以维生素E(VE)为阳性对照药,采用分光光度法检测脑海马组织caspase-3活性变化。结果与假手术组相比,模型组caspase-3活性明显增加(P<0.001);与模型组相比,莫诺苷30mg/kg、90mg/kg、270mg/kg均能够降低caspase-3活性,并呈剂量依赖性(P<0.05);VE(35mg/kg)能显著降低caspase-3活性(P<0.001)。结论莫诺苷能够抑制大鼠脑组织caspase-3活性,通过抗凋亡发挥神经保护作用。Objective To investigate the effects of morroniside on activation of caspase-3 in rats with focal cerebral ischemia/reperfusion.MethodsThe model was induced with occlusion of middle cerebral artery with suture embolus,ischemia for 30 min,and reperfusion for 72 h in rats.Vitamin E used for the positive control.The activity of caspase-3 was detected with spectrophotometry.ResultsCompared with sham group,the caspase-3 activity increased obviously in model rat.Compared with model group,Morroniside（30 mg/kg,90 mg/kg,270 mg/kg）decreased the activation of caspase-3 remarkably,which was dose-dependent（P〈0.05）.ConclusionMorroniside may reduce apoptosis by decreasing the activation of caspase-3 in rats.

关 键 词：莫诺苷 缺血再灌注 CASPASE-3 凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]