依曲替酸上调Fas、FasL诱导皮肤鳞癌细胞凋亡的实验研究  

Acitretin Induces Apoptosis of Cutaneous Squamous Cell Carcinoma Cells by Upregulating Fas and FasL Expression

作　　者：林秀英[1] 韩振东[1] 王佳勇[1] 崔尧[1] 张忠太[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院皮肤科,黑龙江齐齐哈尔161000

出　　处：《中国临床医学》2010年第4期467-469,共3页Chinese Journal of Clinical Medicine

基　　金：黑龙江省自然科学基金资助项目(编号:D2007-110);黑龙江省普通高等学校青年学术骨干支持计划项目(编号:1155G67)

摘　　要：目的:探讨依曲替酸通过上调Fas、FasL基因表达诱导皮肤鳞状细胞癌(鳞癌)细胞系SCC13细胞凋亡的作用。方法:以体外培养的人皮肤鳞癌细胞系SCC13细胞为研究对象,采用酶联免疫分析法(ELISA)、AnnxinV-FITC/PI法检测凋亡的诱导情况。用实时荧光定量RT-PCR方法检测Fas、FasL mRNA的表达。用Fas中和抗体ZB4检测Fas、FasL在依曲替酸诱导SCC13细胞凋亡中的作用。结果:依曲替酸能诱导SCC13细胞凋亡,并具有浓度及时间依赖性。依曲替酸能上调肿瘤细胞内Fas、FasL mRNA表达水平。单独应用依曲替酸的早期凋亡率为(16.76±1.04)%,与ZB4共同处理SCC13后,早期凋亡率下降为(7.12±0.85)%,ZB4显著阻断依曲替酸诱导的凋亡(P<0.05)。结论:依曲替酸能诱导皮肤鳞状细胞癌细胞凋亡,其机制可能与Fas死亡受体信号转导途径有关。Objective：To investigate the effects of acitretin on the apoptosis of human cutaneous squamous cell carcinoma cells by upregulating Fas and FasL expression.Methods： Human cutaneous squamous cell carcinoma cell line SCC13 was treated with acitretin.Apoptosis was assessed by enzyme-linked immunosorbent assay and Annexin V-FITC/PI.The real-time quantitative polymerase chain reaction（RT-PCR） was used to detect the expression of Fas and FasL mRNA.Neutralizing ZB4 anti-Fas antibody was used to analyze the effect of proapoptotic Fas and FasL in SCC13 cells.Results： Acitretin induced apoptosis of SCC13 cells in a dose-and time-dependent manner.Acitretin increased Fas and FasL mRNA level in cancer cells.When treated with acitretin alone,the early apoptosis rate was（16.76±1.04） %,while cotreated with ZB4,it was decreased to（7.12±0.85）%,therefore,ZB4 effectively inhibited the apoptosis induced by acitretin（P0.05）.Conclusions： Acitretin could induce apoptosis of cutaneous squamous cell carcinoma cell line SCC13 by Fas signaling pathway.

关 键 词：依曲替酸 细胞凋亡 癌 鳞状细胞 细胞系 

分 类 号：R739.5[医药卫生—肿瘤]