美托洛尔对异丙基肾上腺素诱导心力衰竭大鼠NE、ANGⅡ的影响  

Effect of metoprolol on changes of plasma NE & Ang Ⅱ in rats with ISO-induced congestive heart failure.

作　　者：蔡辉[1] 郭寒[1] 陆乐[1] 张永文[1] 赵智明[1] 

机构地区：[1]南京军区南京总医院中西医结合科,江苏南京210002

出　　处：《四川医学》2010年第9期1223-1225,共3页Sichuan Medical Journal

基　　金：南京军区南京总医院科研基金资助项目(课题编号:2008042)

摘　　要：目的探讨美托洛尔对充血性心力衰竭大鼠血浆NE、AngⅡ的影响。方法实验选用清洁级雄性SD大鼠60只,随机分为正常对照组(n=10),模型组50只皮下注射异丙肾上腺素2次(170mg/kg),6周后应用超声心动图检测,以左室射血分数≤45%确定造模成功,将心力衰竭大鼠模型随机分为对照组、美托洛尔治疗组8mg/(kg.d),18周使用左心导管进行血流动力学检查,取血利用酶联免疫方法 (ELISA)检测NE和放免法检测血浆AngⅡ,并取心肌观察病理形态学特征。结果①与正常对照组相比,模型组心功能指标明显恶化,血浆NE、ANGⅡ显著升高,心室重量指数显著升高,心室重构明显;②与模型组比较,美托洛尔组心功能明显好转,血浆NE、ANGⅡ明显下降,心室重量指数明显降低,心室重构好转。结论美托洛尔能通过降低心力衰竭大鼠血浆NE、ANGⅡ的浓度,而改善心功能,逆转心室重构。Objective To explore the effect of metoprolol on plasma Renin and Angiotensin Ⅱ in fats with heat failure.Methods 60 Male Sprague-Dawley rats were randomly allocated to the ISO-induced chronic heart falilure group（n=50） receiving two subcutaneous injections of 170mg ISO per-kilogram of body and the healthy normal group（NOR group,n= 10） receiving two subcutaneous injections of 0.25ml normal saline.At 6th week,the left ventricular ejection fraction（LVEF） was measured by echocardiogram,the remaining rats,with an left ventricular ejection fraction≤45%,were randomly divided into two groups： control group（n=10）,metoprolol group 8 mg/（kg·d）（n=10）.the drugs were given i.g.At 18th week,then the hemody namic parameters were detected,including heart rate,left ventricular end diastolic pressure,LV systolic pressure;LVWI was measured after LV morphopathological examination,the Renin and Angiotensin Ⅱ in plasma were detected by ELISA and radio-immunity method,the mass ratio of heart ventricle and left ventricle to body was measured. Results ①Compared with the NOR group,the HR、LVEDP,LVWI and the Renin and Angiotensin Ⅱ in plasma were significantly increased（P0.01）.But LVSP and ±dp/dtmax were significantly decreased in the CON group.②Compared with the CON group,the HR、LVEDP、LVWI and the Renin and AngiotensinⅡin plasma were decreased distinctly.the LVSP and±dp/dtmax were increased distinctly（P0.01） in the MET group.Conclusion Metoprolol can inhibit the Renin and AngiotensinⅡin plasma,inprove heart function and reverse ventricular remodeling with congestive heart failure.

关 键 词：异丙基肾上腺素 美托洛尔 SD大鼠 充血性心力衰竭 RAS 心室重构 

分 类 号：R541.6[医药卫生—心血管疾病]