红曲霉转化中药葛根转化产物的提取工艺研究  

Study on the Extraction Technology for Transforming Puerariae Radix with Monascus purpureus

作　　者：宋艳秋[1] 陈有为[2] 

机构地区：[1]大理学院公共卫生学院,云南大理671000 [2]云南大学省微生物研究所,云南昆明650091

出　　处：《安徽农业科学》2010年第19期10476-10477,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究红曲霉YM3207对葛根进行微生物转化后,其转化产物的最佳提取工艺。[方法]以葛根素含量为指标,HPLC法为测定方法,采用单因素和正交试验优化提取工艺。[结果]选用超声波提取,最佳提取工艺条件:以70%乙醇为提取剂,乙醇体积为发酵产物质量的11倍,超声提取3次,每次超声时间40min。[结论]用选定的工艺,转化产物中葛根素提取效率高,结果稳定。[Objective] The aim was to screen out the optimum extraction technology for transforming Puerariae radix with Monascus purpureus.[Method] Taking puerarin content as the index,HPLC was adopted and orthogonal test was used in this experiment.[Result] The best extraction conditions were as follows：adding 11 times of 70% alcohol,ultrasonic extracting 40 min for 3 times.[Conclusion] The optimal condition is efficient and stable.

关 键 词：红曲霉 葛根 提取工艺 

分 类 号：R284.2[医药卫生—中药学]