PCNA在大鼠肠缺血再灌注损伤中的表达及意义  

Research on Dynamic Alteration of proliferating cell noclear antigen on the Model of Intestinal Ischemia Reperfusion In jury in Rats

作　　者：申志新[1] 高海玲[2] 孙伟[1] 

机构地区：[1]潍坊医学院临床医院,261031 [2]潍坊医学院

出　　处：《中国科技信息》2010年第18期214-216,共3页China Science and Technology Information

摘　　要：目的探讨小肠缺血再灌注后不同时限小肠黏膜中增殖细胞核抗原(PCNA)的动态变化。方法选用健康雄性SD大鼠,建立小肠缺血再灌注损伤模型。每组大鼠根据缺血再灌注后不同时限,分为对照组、缺血再灌注后0min,30min,1h,2h,6h,12h,24h,48h,72h共10组(每组6只)。在缺血再灌注后不同时间点,采用HE染色观察小肠黏膜组织学形态,TUNEL染色、免疫组织化学方法检测PCNA的表达。结果①大鼠小肠历经热缺血40min后,再灌注0～72h大致经历了潜伏期、急性期、交错期、恢复期4个阶段的形态变化。缺血再灌注后,出现明显的细胞凋亡及组织学病理改变,开始于潜伏期,加重于急性期。②与对照组相比,PCNA在缺血再灌注组表达增强(P<0.05)。结论①小肠在短时间缺血后,再灌注可出现明显的细胞凋亡及组织学病理改变。大多数小肠黏膜细胞经历短暂的凋亡前期反应后则进入恢复阶段,最终结果是小肠黏膜结构和功能的修复。②PCNA参与了小肠缺血再灌注损伤的病理生理过程,可作为动态监测机体缺血再灌注损伤中细胞凋亡与增殖的有效指标。Objective To provide experiment evidence for treating the intestine damage induced by ischemia reperfusion in intestinal surgical operation in rats.Methods Intestinal ischemia reperfusion injury models were established in SD rats.According to different ischemic and reperfusive time,animals were divided into ischemia reperfusion groups and control groups.The reperfusion was performed 40minutes after ischemia in the intestines of the SD rats.The intestinal tissues of the experimental groups were taken at 0min,30min,1h,2h,6h,12h,24h,48h,and 72h respectively after the reperfusion and the control groups were treated with pseudooperation.The mucosa epithelial cells apoptosis and proliferation induced by ischemia reperfusion were dynamically determined by TUNEL and electron microscope,and immunohistochemistry to detect PCNA.Results ①The rat intestines were undergone 4 stage changes as incubation,acute,alternative and recovery stages from 40 minutes after ischemia to 72 hours after reperfusion.The epithelial cells apoptosis and histopathologic changes induced by ischemia reperfusion began at the incubation stage,aggravated at the acute stage.②The expression of PCNA was enhanced in I/R groups.The difference of expression of PCNA between the two groups was significant at the same time point（P 0.05）.Conclusions ① It induced an obviously epithelial cells apoptosis and histopathologic changes after a short time ischemia and reperfusion.Most cells turned into convalescence after a short pre-apoptosis reaction,then the intestine mucosal structure and function were repaired.②PCNA contributed to intestine ischemia reperfusion,and could be one of valued index to the dynamic alteration of intestine ischemia reperfusion.

关 键 词：大鼠 缺血再灌注损伤 增殖细胞核抗原 细胞凋亡 细胞增殖 

分 类 号：R363[医药卫生—病理学]