端脑素在缺氧缺糖环境中对PAJU细胞的保护作用  

作　　者：杨和平[1,2] 彭熠[2,3] 杨力强[4] 苏上贵[5] 农田泉[1] 

机构地区：[1]广西中医学院附属瑞康医院神经内科 [2]中南大学湘雅二医院神经内科 [3]湖南省人民医院神经内科 [4]广西中医学院药理教研室 [5]广西医科大学生物化学教研室

出　　处：《中国科技信息》2010年第18期217-219,共3页China Science and Technology Information

摘　　要：目的:探索端脑素在缺氧缺糖环境中是否对神经元具有保护作用。方法:建立直接氧糖剥夺(OGD)神经元模型并验证OGD模型的效果;以稳定表达常量人端脑素蛋白的PAJU-TLN细胞株和具有NEO抗性的PAJU-NEO细胞株作为神经元OGD模型,分别对PAJU-TLN和PAJU-NEO细胞进行OGD处理,用流式细胞仪检测细胞凋亡率。结果:经免疫荧光检测缺氧诱导因子HIF-1a的表达证实OGD神经元模型有效,经过OGD处理后,PAJU-TLN细胞凋亡率明显较PAJU-NEO细胞降低(P<0.05)。结论:端脑素在OGD环境中可以减轻OGD诱导的细胞凋亡,对于缺氧缺糖环境中的神经细胞可能具有保护作用。Objective：In this study we investigated the protective effects of telencephalin（TLN） in oxygen-glucose deprivation（OGD） neuronal model.Methods：Establishment of neuron oxygen glucose deprivation（OGD） model in vitro by using PAJU-TLN cell line which express normal TLN and PAJU-NEO cell line which express neo resistant as neuronal models.Both PAJU-TLN and PAJU-NEO cells were treated in OGD in the same times respectively.Flow cytometer（FCM） was used to exam apoptosis of both treated PAJU-TLN and PAJU-NEO cells.Results：The OGD model was established and verified by expression of hypoxia inducible factor1 α（HIF-1 α）.The apoptosis rate of PAJUTLN cells is lower than that of PAJU-NEO cells after both cell lines were treated in OGD in the same time.Conclusions：PAJU cells which express of TLN may protect neurons from oxygen-glucose deprivation injury.

关 键 词：端脑素 氧糖剥夺 神经保护 

分 类 号：R743.3[医药卫生—神经病学与精神病学]