大豆异黄酮对豚鼠乳头肌动作电位和心肌细胞钠电流的影响  

Effects of soybean isoflavone on action potential of guinea pig papillary muscle and sodium current in cultured neonatal rat cardiomyocyte

作　　者：马丽娟[1] 张文杰[2] 张雨薇[2] 赵春燕[2] 

机构地区：[1]浙江医学高等专科学校基础医学部解剖教研室,杭州310053 [2]吉林大学基础医学院生理教研室

出　　处：《中华老年医学杂志》2010年第8期679-682,共4页Chinese Journal of Geriatrics

基　　金：吉林省科委自然科学基金（20020503-16）;浙江省教育厅科研基金（201006）

摘　　要：目的 观察大豆异黄酮（soybean isoflavone,SI）对豚鼠乳头肌动作电位的影响和培养心室肌细胞钠电流的作用.方法 采用悬浮玻璃微电极法测定乳头肌的动作电位和全细胞膜片钳方法测定心室肌细胞的钠电流.结果 SI能剂量依赖性地降低乳头肌动作电位幅值（APA）,且能同时缩短复极50%及90%水平的动作电位时程（APD50、APD90）;SI 0.1μg/ml和1.0μg/m1对心室肌细胞钠电流抑制率是14.39%和23.48%.结论 SI对心肌有负性肌力作用,可使心肌细胞动作电位除极参数降低,动作电位时程缩短,对钠电流的抑制是其负性肌力作用和抗心律失常作用的离子机制之一.Objective To observe the effects of soybean isoflavone （SI） on the action potential of guinea pig papillary muscle and the sodium current in cultured neonatal rat cardiomyocyte.Methods The action potential of guinea pig papillary muscle was measured by glass suspension electrode and the sodium current in cultured neonatal rat cardiomyocyte was measured by whole cell patch-clamp technique. Results The SI reduced the action potential amplitude （APA） and shortened the 50% and 90% of duration of action potential （APD50 and APD90）. The SI 0.1μg/ml inhibited sodium current in cardiomyocyte at a inhibition rate of 14.39 %, and the corresponding data of SI 1.0μg/ml at a inhibition rate of 23.48%. Conclusions The SI can reduce the APD of myocardiocyte and has a negative inotropic action on myocardium. These results indicate that SI inhibition of the sodium current may be one of mechanisms of its antiarrhythmic action.

关 键 词：异黄酮类 肌细胞 心脏 动作电位 钠通道 豚鼠 

分 类 号：R[医药卫生]