E-选择素及其配体SLeX介导大肠癌早期黏附的观察  

Effects of E-selectin and its ligand SLeX in mediating early adhesion of colorectal carcinoma

作　　者：周萍[1] 成玉霞[1] 张贵慧[1] 孙青[1] 

机构地区：[1]山东省千佛山医院病理科,山东济南250014

出　　处：《中华肿瘤防治杂志》2010年第16期1273-1276,1279,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省医药卫生科研项目(HZ117)

摘　　要：目的:探讨E-选择素及其配体SLeX在大肠癌LoVo细胞与血管内皮细胞ECV304早期黏附中的作用。方法:采用SP法观察E-选择素在内皮细胞ECV304及LoVo中的表达;采用活性染料玫瑰红摄入法检测E-选择素及其配体SLeX在大肠癌LoVo细胞与TNF-α激活内皮细胞ECV304早期黏附中作用。结果:E-选择素和SLeX分别在ECV304和LoVo细胞膜及细胞质内有明确的阳性表达;血管内皮细胞ECV304被TNF-α激活后,其与LoVo细胞间的黏附较激活前明显增加,且具有浓度及时间的依赖性,P<0.001;用不同浓度的抗E-选择素单抗处理血管内皮细胞ECV304,或用其配体SLeX单抗处理肿瘤Lo-Vo细胞后,随着E-选择素单抗、SLeX单抗浓度增加,细胞相对黏附率逐渐降低(P<0.001),阻断率逐渐增加(P均<0.05),提示E-选择素单抗、SLeX单抗可阻断大肠癌细胞与血管内皮细胞间黏附。结论:E-选择素和其配体SLeX是介导血管内皮细胞与大肠癌Lo-Vo细胞系黏附反应的主要早期黏附分子。OBJECTIVE：To study the role of E-selectin and SLeX in the adhesion between colorectal carcinoma LoVo cells and endothelial cell ECV304.METHODS：Using the immunocytochemisty method of strepto-avidin-biotin peroxidase complex （SP） detected the expression of E-selectin in human endothelial ECV304 cells;The adhesion of LoVo cells to human umbilical vein endothelial cells （ECV304） was detected by tumor cells uptaking Rose Bengal.RESULTS：The expression of E-selectin in Human umbilical vein endothelial cells （ECV304） and SLeX in LoVo cells were observed;After ECV304 cells were stimμLated by TNF-α,the adhesion of LoVo cells to ECV304 was more increased （P0.001）,and the effect was time and TNF-αdensity dependent;After LoVo cells and ECV304 cells were exposed to different anti-E-selectin and anti-SLeX mAbs concentrences respectively,the cell relative adhesion rate decreased with anti-E-selectin and anti-SLeX mAbs concentrences（P0.001）.The cell adhesion between LoVo and ECV304 coμLd be blocked by anti-E-selectin and anti-SLeX mAbs（P0.05）.CONCLUSION：E-selectin and its ligand SLeX play an important role in mediating the adhesion reaction between LoVo cells and vascμLar endothelial cells.

关 键 词：肠肿瘤 E-选择素 细胞黏附/药物作用 

分 类 号：R735.34[医药卫生—肿瘤]