Effect of Matrine on Human Ether à go-go Related Gene (HERG) Channels Expressed in Chinese Hamster Ovary Cells  

作　　者：吴红金 邹安若 谢芳 杜以梅 曹于 刘宇娜 杨继媛 李新民 

机构地区：[1]Beijing Hospital of Integrated Traditional Chinese and Western Medicine [2]Ion Channelopathy Research Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

出　　处：《Chinese Journal of Integrative Medicine》2010年第5期430-434,共5页中国结合医学杂志（英文版）

基　　金：Supported by Beijing Administration Bureau of Traditional Chinese Medicine (No. JZZ-312)

摘　　要：Objective： To observe the effect of matrine on human ether à go-go related gene （HERG） potassium channels expressed in Chinese hamster ovary （CHO） cells and investigate whether HERG channel is a new target of the pharmacological effect of matrine on arrhythmia and tumor. Methods： HERG channel potassium current in CHO cell was recorded using whole-cell patch-clamp technique, and the influence of matrine on the current was explored. Results： Matrine inhibited HERG potassium current in a dose-dependent manner, and the 50% inhibitory concentration （IC50） was 411±23 μmol/L. Matrine had no significant effect on the activation kinetics, and mainly blocked HERG channels in their closed state. Conclusions： The blocking effect of matrine on HERG channels might be one of the mechanisms against arrythmias and tumors. Unlike most other blockers exerting blocking effect at the intracellular sites by entering the cell with the opening of HERG channel, matrine blocked HERG channels at the extracellular sites.Objective： To observe the effect of matrine on human ether à go-go related gene （HERG） potassium channels expressed in Chinese hamster ovary （CHO） cells and investigate whether HERG channel is a new target of the pharmacological effect of matrine on arrhythmia and tumor. Methods： HERG channel potassium current in CHO cell was recorded using whole-cell patch-clamp technique, and the influence of matrine on the current was explored. Results： Matrine inhibited HERG potassium current in a dose-dependent manner, and the 50% inhibitory concentration （IC50） was 411±23 μmol/L. Matrine had no significant effect on the activation kinetics, and mainly blocked HERG channels in their closed state. Conclusions： The blocking effect of matrine on HERG channels might be one of the mechanisms against arrythmias and tumors. Unlike most other blockers exerting blocking effect at the intracellular sites by entering the cell with the opening of HERG channel, matrine blocked HERG channels at the extracellular sites.

关 键 词：MATRINE patch-clamp technique human ether à go-go related gene potassium channel Chinese hamster ovary cell 

分 类 号：R285[医药卫生—中药学]