高三尖杉酯碱和血栓通对人翼状胬肉成纤维细胞周期性变化的影响  

A study on effect of homoharringtonine and xueshuantongon cell cycle of fibroblasts derived from pterygium

作　　者：罗晨美[1,2,3] 王映芬[1,2,3] 黄培根[1,2,3] 蔡康荣 龙心光[1,2,3] 陈毓东 

机构地区：[1]广东医学院微生物免疫学教研室 [2]广东医学院附属医院眼科 [3]广东医学院病理学教研室

出　　处：《中国中医眼科杂志》1999年第2期67-70,共4页China Journal of Chinese Ophthalmology

基　　金：广东省卫生厅科研基金

摘　　要：目的：研究高三尖杉酯碱和血栓通对培养人翼状胬肉成纤维细胞周期性变化的影响。方法：对翼状胬肉组织中的成纤维细胞进行培养并传代为第３代～５代。用高三尖杉酯碱（１０－５ｍｏｌ／Ｌ）及血栓通（１．５ｇ／Ｌ）分别处理２４ｈ，对照组用生理盐水。应用ＰＩＤＮＡ荧光染色及流式细胞仪检测和分析细胞周期的变化。结果：高三尖杉酯碱组和血栓通组Ｇ１群体细胞明显增多，Ｓ期细胞明显减少。与对照组比较差异均有明显意义（Ｐ＜０．０１），其中高三尖杉酯碱更为明显。结论：高三尖杉酯碱和血栓通对翼状胬肉成纤维细胞的增生均有明显抑制作用，以高三尖杉酯碱更显著。其作用机理可能与抑制细胞ＤＮＡ合成有关。OBJECTIVE: To study the effect of homoharringtonine and xue shuan tong on cell cycle of fibroblasts cultivated from human pterygium. METHODS: The fibroblasts derived from 3 to 5 generations of the cultured pterygium tissues. These cells had been treated with homoharringtonine (10 -5 mol/L) and xue shuan tong1 5 g/L respectively, in the control group the cells had been treated with normal saline. The changes of cell cycle were observe and detected cells by the methods of propidium iodide (PI) staining and flow cytometry (FCM). RESULTS:Compared with the control group, the distribution in cell cycle was showed that the percentage of G1 cells was increased, and the S phase cells were decreased in the homoharringtonine and xue shuan tong treated groups respectively. There was statistical significant between the experimental group and control groups( P <0 01), and the effect of homoharringtonine group was more obvious. CONCLUSIONS:The homoharringtonine and xue shuan tong can significantly inhibit the proliferation of the fibroblasts derived from pterygium. The mechanism of effect could be related with inhibition of synthesizing DNA.

关 键 词：高三尖杉酯碱 血栓通 治疗 成纤维细胞 翼状胬肉 

分 类 号：R777.330.5[医药卫生—眼科]