腺样囊性癌嗜神经侵袭中神经生长因子、上皮钙黏素和钙结合蛋白A4的表达及意义  

Effect of nerve growth factor on adhesion and motional abilities of tumor cells and their correlation

作　　者：骆树瑜[1] 张剑明[1] 李宏捷[1] 郑善川[1] 张玉茹[1] 朱晓虹[1] 

机构地区：[1]天津医科大学口腔医院病理科,300070

出　　处：《中华口腔医学杂志》2010年第9期531-534,共4页Chinese Journal of Stomatology

基　　金：教育部留学回国人员科研启动基金（2005383）

摘　　要：目的 探讨腺样囊性癌（adenoid cystic carcinoma,ACC）组织中神经生长因子（nerve growth factor,TGF）及上皮钙黏素（E-cadherin,E-cad）、钙结合蛋白A4（S100A4）的表达和临床意义,分析它们之间的相关性及其与ACC嗜神经侵袭（perineural invasion,PNI）现象的关系,进一步探讨NGF在PNI过程中对ACC细胞的黏附力和迁移运动能力的影响.方法 对42例ACC组织按年龄、性别、发病部位、临床分期及PNI和非PNI（NPNI）进行分组,42例中PNI组26例,NPNI组16例,应用免疫组化SP法检测其中NGF、E-cad和S100A4的表达与分布,分析三者与临床病理参数之间的关系,并对它们在不同病理类型和组织学部位的表达进行统计学分析.结果 NGF和S100A4在PNI组的表达率分别为88%（23/26）和77%（20/26）,较NPNI组（8/16和7/16）高,差异有统计学意义（P＜0.05）,两者在PNI组的表达呈正相关（r=0.316,P＜0.05）.PNI组中E-cad的表达为31%（8/26）,较NPNI组（12/16）低（P＜0.05）,与NGF的表达（r=-0.385,P＜0.05）和S100A4的表达呈明显负相关（r=-0.612,P=0.000）.NGF在PNI组ACC神经周组织的表达[83%（25/30）]高于远离神经部位[47%（14/30）],差异有统计学意义（P＜0.05）.结论 在ACC嗜神经侵袭过程中,NGF可能扮演着重要角色,但并非惟一因素,NGF可能通过和相应受体结合提高S100A4的表达率而降低E-cad 的表达和生物活性,使癌细胞间黏附力降低,运动能力增强进而促进ACC对神经组织的侵袭.Objective To investigate the effect on adhesion and motion capbilities of adenoid cystic carcinoma（ACC） , by detecting the expression of nerve growth factor （NGF） , E-cadherin （E-cad） and S100A4 and the clinical significance in ACC tissues and analyzing the relationships between them with perineural invasion（PNI）. Methods The expression of NGF, E-cad and S100A4 in ACC was detected with the way of immunohistochemistry SP, and then analyzing the expression level of them in different pathological types and histological regions in statistical ways on the basis of their relation with clinical and pathological parameters. Results The expression of NGF and S100A4 in PNI group [ 88% （23/26） and 77% （20/26） ] , was higher than that in NPNI group （8/16 and 7/16, P 〈0.05）, and a positive correlation between them was identified in PNI group（r =0. 316,P 〈0.05）. However the E-cad expression was lower in PNI group[31%（8/26） ,P〈0. 05]. On the other hand it suggested a negative correlation with NGF （r = 0.385, P〈0.05） as well as that with S100A4（r = -0.612,P =0.000）. The expression level of NGF in fasciculus[ 83% （25/30）] has significant deviation compared with it in distant tumor tissues [ 47% （14/30）, P〈0. 05]. Conclusions In the PNI process of ACC, NGF plays important parts but not the only factor. It can increase the expression and activity of S100A4 but decrease E-cad expression through binding with its receptor. Thus, adhesion abilities of tumor cells was weakened and motional abilities was strengthen.

关 键 词：神经生长因子 钙黏着糖蛋白类 癌 腺样囊性 嗜神经侵袭 

分 类 号：R739.8[医药卫生—肿瘤]