一种改进的更为稳定可靠的远端大脑中动脉闭塞模型  

An improved, stable and reliable distal middle cerebral artery occlusion model evaluated by microPET, microMRl, TTC staining, CV staining and behavioral test

作　　者：任传成[1] 杨立刚[2] 靳峥[2] 章军建[1] 

机构地区：[1]武汉大学中南医院神经科,430071 [2]复旦大学附属上海市第五人民医院神经科

出　　处：《中华神经科杂志》2010年第9期649-654,共6页Chinese Journal of Neurology

摘　　要：目的建立一种稳定可靠的脑缺血研究模型。方法（1）远端大脑中动脉闭塞（dMCAO）模型的制作：SD大鼠（270—350g），异氟烷麻醉，永久阻断左侧大脑中动脉远端并夹闭双侧颈总动脉30min即制作成功dMCAO模型。（2）模型的评定：分别于术后10h行PET扫描、术后24h行MRI扫描；术后48h行1Trc染色进行梗死面积的评定；分别于术前，术后1、2、7、10、14、21、30、37、44、51、60d进行行为学评定；CV染色评定细胞受损情况；并计算死亡率。结果MRI测定梗死面积为45．30％±2．35％（95％可信限：42．94～47．86），TTC测得梗死面积为43．39％±2．33％（95％可信限：40．94～45．84），PET测得缺血面积为54．50％±3．15％（95％可信限：49．49～59．51）。术后60dCV染色测得梗死面积为30．10％±2．22％（95％可信限：28．05—32．15），行为学评定：基线评分两组无差异，60d总评分差异有统计学意义，在术后各时间点梗死组与对照组相比差异也有统计学意义。模型制作过程中无个体排除，无死亡。结论30min一过性阻断双侧颈总动脉加永久性阻断左侧大脑中动脉远端所产生的dMCAO模型稳定、可靠，适用于脑缺血研究。Objective To create a stable and reliable model for cerebral ischemia. Methods （ 1 ） Distal middle cerebral artery occlusion （dMCAO） model： SD rats of 270--350 g in weight were anesthetized using isoflurane. Both common carotid arteries （CCA） were exposed and occluded for 30 min. Via a bone window between the left eye and ear, the exposed left middle cerebral artery was cauterized and cut during bilateral CCA occlusion. （2） Evaluation of the model： microPET study was performed at 10 h after surgery. microMRI scan were done at 24 h. TTC staining were done at 48 h. Behavioral tests, including vibrissaeelicited forelimb placement test, were done from day 2 to 60 after surgery. Tissue damage was evaluated using cresyl-violet staining. Mortality was also observed. Results Infarction areas were 54. 50% ± 3.15% （95% CI：49.49-59. 51 ） using microPET scanning, 45.30% ± 2. 35% （95% CI：42. 94-47. 86） using microMRI scanning, 43.39% ± 2. 33% （95% CI：40. 94-45.84） using TTC staining, and 30. 10% ±2. 22% （95% CI：28.05-32. 15） using CV staining. The behavioral test scores were lower in the ischemic group than in the sham control group . This dMCAO model was successfully performed in all rats, and the mortality rate was 0. Conclusions Our results suggest that permanent dMCAO plus bilateral CCA occlusion for 30 min can produce a stable and reliable model suitable for research on cerebral ischemia.

关 键 词：梗死 大脑中动脉 脑缺血 疾病模型 动物 

分 类 号：R743.3[医药卫生—神经病学与精神病学]