秋葵幼胚培养试验初步研究  

Preliminary Study on Immature Embryo Culture of Okra

作　　者：张坤亮[1] 何道一[1] 

机构地区：[1]淮北师范大学生命科学学院资源植物生物学安徽省重点实验室,安徽淮北235000

出　　处：《淮北煤炭师范学院学报（自然科学版）》2010年第3期37-39,共3页Journal of Huaibei Coal Industry Teachers College(Natural Science edition)

基　　金：安徽省资源植物学重点实验室资助项目(2009015)

摘　　要：以秋葵幼胚为试材,筛选较适合幼胚萌发和成苗的培养基,并研究胚龄分别为开花后4 d、6 d、8 d、10 d的幼胚萌发率和成苗率.结果表明,秋葵幼胚萌发和成苗的适宜培养基分别为Qz(Ms+0.1 mg/mL BA+1.0 mg/mL GA3)和QG(Ms+0.5mg/mL GA3);开花后8 d的幼胚萌发率和成苗率最高.由此建立秋葵幼胚培养的方案.Immature embryos of okra were used to screen the optimum medium of germination and seedling.Embryos with different developing stage（4,6,8 and 10 days after florescence） were investigated in their germinating rate and seedling rate.The results showed that Qz（Ms ＋ 0.1 mg / mL BA ＋ 1.0 mg /mL GA3） was the effective medium of germination and QG（Ms ＋ 0.5 mg /mL GA3） was the effective medium of seedling.Immature embryos after flowering 8 d have highest germinating and seedling rate.The protocol for immature embryo culture of okra was established.

关 键 词：秋葵 幼胚 萌发率 成苗率 

分 类 号：Q943.1[生物学—植物学]