枸杞多糖对THP-1单核细胞源性泡沫细胞形成的影响  

The Effect of Lycium Barbarum Poysaccharides on the Formation of THP-1 Monocyte-derived Foam Cells and Cholesterol Lipid Outflow

作　　者：李煜淇[1] 韩学波[1] 杨安宁[1] 姜怡邓[1] LI Yu-qi,HAN Xue-bo,YANG An-ning,JIANG Yi-deng （Ningxia Medical University,Yinchuan 750004,China）

机构地区：[1]宁夏医科大学,宁夏银川750004

出　　处：《医学信息》2010年第20期3764-3765,共2页Journal of Medical Information

基　　金：宁夏医科大学特殊人才项目（XT200803）; 宁夏自治区卫生厅重点科研计划（建议）项目（W200720）

摘　　要：目的探讨枸杞多糖对THP-1单核细胞源性泡沫细胞形成和胆固醇脂流出的影响。方法将THP-1单核细胞与氧化低密度脂蛋白（ox-LDL）、佛波酯（PMA）、枸杞多糖共同培养,油红O染色检测泡沫细胞的形成,同时测定泡沫细胞中SOD、GSH-Px、T-AOC活性和MDA的含量,并检测枸杞多糖对单核细胞源性泡沫细胞胆固醇脂流出的影响,观察枸杞多糖对THP-1单核细胞泡沫化形成的影响及可能机制。结果枸杞多糖抑制了ox-LDL和PMA激活的THP-1单核细胞内脂质过氧化产物的产生、胆固醇脂的积聚和泡沫细胞的形成。结论枸杞多糖对泡沫细胞的形成和胆固醇脂流出有一定的抑制作用,其抗氧化作用是重要机制之一。Objective To investigate the effect of Lycium barbarum poysaccharides on the formation of THP-1 monocyte-derived foam cells and cholesterol lipid outflow.Methods co-cultured THP-1、ox-LDL、PMA and LBP with the THP-1 monocyte,to detect the formation of foam cell by oil red O stained,and determinat SOD,GSH-Px,T-AOC activity of foam cells and MDA content.to measure the effect of LBP on the THP-1 monocytederived foam cells formation and cholesterol lipid influence,in order to observe the effect and possible mechanisms of LBP on the formation of THP-1 monocyte-derived foam cells.Results LBP inhibited lipid the accumulation of peroxidation,cholesterol and the formation of foam cells which actived by ox-LDL and PMA.Conclusion LBP play an important role on the formation of foam cells and cholesterolinfluence,the antioxidant of LBP is one of the important mechanisms.

关 键 词：枸杞多糖 泡沫细胞 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学;医药卫生—基础医学]